High release of Candida albicans eDNA as protection for the scaffolding of polymicrobial biofilm formed with Staphylococcus aureus and Streptococcus mutans against the enzymatic activity of DNase I.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Santiago M Lattar, Rene Peter Schneider, Vidal Jorge Eugenio, Gabriel Padilla
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引用次数: 0

Abstract

This study aimed to determine the protective role of the high release of C. albicans extracellular DNA (eDNA) in a polymicrobial biofilm formed by S. aureus and S. mutans in the course of DNase I treatment. A tube-flow biofilm bioreactor was developed to mimic biofilm formation in the oral cavity. eDNA release was quantified by real-time PCR (qPCR) and confocal microscopy analysis were used to determine the concentration and distribution of eDNA and intracellular DNA (iDNA). The mean amount of eDNA released by each species in the polymicrobial was higher than that in monospecies biofilms (S. aureus: 3.1 × 10-2 ng/μl polymicrobial versus 5.1 × 10-4 ng/μl monospecies; S. mutans: 3 × 10-1 ng/μl polymicrobial versus 2.97 × 10-2 ng/μl monospecies; C. albicans: 8.35 ng/μl polymicrobial versus 4.85 ng/μl monospecies). The large amounts of eDNA released by C. albicans (96%) in polymicrobial biofilms protects the S. aureus and S. mutans cells against the degradation by DNase I and dampens the effect of clindamycin.

白色念珠菌 eDNA 的高释放量可保护与金黄色葡萄球菌和变异链球菌形成的多微生物生物膜支架免受 DNase I 酶活性的破坏。
本研究旨在确定在金黄色葡萄球菌和变异葡萄球菌形成的多微生物生物膜中,白僵菌胞外DNA(eDNA)的大量释放在DNase I处理过程中的保护作用。通过实时定量 PCR(qPCR)和共聚焦显微镜分析确定 eDNA 和细胞内 DNA(iDNA)的浓度和分布。与单菌种生物膜相比,多菌种生物膜中各菌种释放的 eDNA 的平均量更高(金黄色葡萄球菌:3.1 × 10-2 ng/μl 多菌种生物膜对 5.1×10-4纳克/微升单菌种;变异杆菌:3×10-1纳克/微升多菌种对2.97×10-2纳克/微升单菌种;白僵菌:8.35纳克/微升多菌种对4.85纳克/微升单菌种)。白僵菌在多微生物生物膜中释放的大量 eDNA(96%)可保护金黄色葡萄球菌和变异金黄色葡萄球菌细胞不被 DNase I 降解,并抑制克林霉素的作用。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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