Polyethylene microplastics affect behavioural, oxidative stress, and molecular responses in the Drosophila model.

IF 4.3 3区 环境科学与生态学 Q1 CHEMISTRY, ANALYTICAL
Himanshu Ranjan, Swetha Senthil Kumar, Sharine Priscilla, Subhashini Swaminathan, Masakazu Umezawa, Sahabudeen Sheik Mohideen
{"title":"Polyethylene microplastics affect behavioural, oxidative stress, and molecular responses in the <i>Drosophila</i> model.","authors":"Himanshu Ranjan, Swetha Senthil Kumar, Sharine Priscilla, Subhashini Swaminathan, Masakazu Umezawa, Sahabudeen Sheik Mohideen","doi":"10.1039/d4em00537f","DOIUrl":null,"url":null,"abstract":"<p><p>The escalating presence of microplastic pollution poses a significant environmental threat, with far-reaching implications for both ecosystems and human health. This study investigated the toxicological impact of polyethylene microplastics (PE MPs) using <i>Drosophila melanogaster</i>, fruit flies, as a model organism. <i>Drosophila</i> were exposed to PE MPs orally at concentrations of 1 mg ml<sup>-1</sup> and 10 mg ml<sup>-1</sup> agar food. The study assessed behavioural parameters and biochemical markers including reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione-<i>S</i>-transferase (GST) activity. The expression levels of key genes (<i>Hsp70Bc</i>, <i>rpr</i>, and <i>p53</i>) were also analysed using the RT-qPCR technique. Results indicated a significant decline in climbing activity among adult flies and crawling behaviour in larvae, indicating potential disruption of motor function. Biochemical analysis revealed elevated ROS levels, indicative of oxidative stress, in both larval and fly stages. Moreover, the antioxidant defence system exhibited decreased SOD activity and a concentration-dependent increase in GST activity indicating the functioning of a quick xenobiotic clearance mechanism. Gene expression analysis demonstrated upregulation of <i>rpr</i>, <i>p53</i>, and <i>Hsp70Bc</i> genes, suggesting activation of cell death pathways and stress response mechanisms. Overall, these findings underline the adverse effects of PE MPs on <i>Drosophila</i>, including behavioural impairment, oxidative stress, and activation of stress response pathways.</p>","PeriodicalId":74,"journal":{"name":"Environmental Science: Processes & Impacts","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science: Processes & Impacts","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1039/d4em00537f","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

The escalating presence of microplastic pollution poses a significant environmental threat, with far-reaching implications for both ecosystems and human health. This study investigated the toxicological impact of polyethylene microplastics (PE MPs) using Drosophila melanogaster, fruit flies, as a model organism. Drosophila were exposed to PE MPs orally at concentrations of 1 mg ml-1 and 10 mg ml-1 agar food. The study assessed behavioural parameters and biochemical markers including reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione-S-transferase (GST) activity. The expression levels of key genes (Hsp70Bc, rpr, and p53) were also analysed using the RT-qPCR technique. Results indicated a significant decline in climbing activity among adult flies and crawling behaviour in larvae, indicating potential disruption of motor function. Biochemical analysis revealed elevated ROS levels, indicative of oxidative stress, in both larval and fly stages. Moreover, the antioxidant defence system exhibited decreased SOD activity and a concentration-dependent increase in GST activity indicating the functioning of a quick xenobiotic clearance mechanism. Gene expression analysis demonstrated upregulation of rpr, p53, and Hsp70Bc genes, suggesting activation of cell death pathways and stress response mechanisms. Overall, these findings underline the adverse effects of PE MPs on Drosophila, including behavioural impairment, oxidative stress, and activation of stress response pathways.

聚乙烯微塑料影响果蝇模型的行为、氧化应激和分子反应。
微塑料污染的不断加剧对环境构成了严重威胁,对生态系统和人类健康都有深远影响。本研究以黑腹果蝇为模式生物,研究了聚乙烯微塑料(PE MPs)的毒理学影响。果蝇口服暴露于浓度为 1 毫克毫升-1 和 10 毫克毫升-1 的琼脂食物中的聚乙烯微塑料。研究评估了行为参数和生化指标,包括活性氧(ROS)、超氧化物歧化酶(SOD)和谷胱甘肽-S-转移酶(GST)活性。此外,还利用 RT-qPCR 技术分析了关键基因(Hsp70Bc、rpr 和 p53)的表达水平。结果表明,成蝇的攀爬活动和幼虫的爬行行为明显下降,表明运动功能可能受到破坏。生化分析表明,幼虫和苍蝇阶段的 ROS 水平升高,表明存在氧化应激。此外,抗氧化防御系统显示 SOD 活性降低,而 GST 活性随浓度增加,这表明异生物快速清除机制正在发挥作用。基因表达分析表明 rpr、p53 和 Hsp70Bc 基因上调,表明细胞死亡途径和应激反应机制被激活。总之,这些发现强调了 PE MPs 对果蝇的不利影响,包括行为障碍、氧化应激和应激反应途径的激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Environmental Science: Processes & Impacts
Environmental Science: Processes & Impacts CHEMISTRY, ANALYTICAL-ENVIRONMENTAL SCIENCES
CiteScore
9.50
自引率
3.60%
发文量
202
审稿时长
1 months
期刊介绍: Environmental Science: Processes & Impacts publishes high quality papers in all areas of the environmental chemical sciences, including chemistry of the air, water, soil and sediment. We welcome studies on the environmental fate and effects of anthropogenic and naturally occurring contaminants, both chemical and microbiological, as well as related natural element cycling processes.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信