Control over membrane fluidity and biophysical properties of synthetic terpolymer stabilized complex coacervates†

IF 4.1 2区 化学 Q2 POLYMER SCIENCE
Sebastian Novosedlik , Alexander B. Cook , Tim J. F. M. Voermans , Henk M. Janssen , Jan C. M. van Hest
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Abstract

Cell membranes are vital barriers that regulate the composition of the intracellular environment and facilitate communication processes essential for cellular function and survival. In comparison to lipid membranes, artificial polymeric membranes generally offer enhanced stability due to their higher molecular weight and greater variability in the nature of the macromolecular building blocks, which provides access to a broad chemistry toolbox to regulate important features such as fluidity and permeability. We recently developed an artificial cell platform based on a complex coacervate, in which a terpolymer, composed of a hydrophilic poly(ethylene glycol) segment, a hydrophobic poly(caprolactone-g-trimethylene carbonate) domain and a polyglutamate anchor (PEG-PCLgTMC-PGA) was used for stabilization. These membranized structures showed excellent permeability, due to the high fluidity of the membrane. However, the polymer membrane proved to be unselective with regard to the molecular weight of guest molecules that were exchanged with the environment. To advance this platform, a series of terpolymers with distinctive features were synthesized to further refine their regulatory features of the polymer membrane. Through investigation of structural terpolymer variants, including those in which the hydrophobic domain was based on PCLgTMC, poly(d,l-lactic acid) or polystyrene, their influence on membrane permeability, fluidity, and sequestration of hydrophobic molecules, such as cholesterol, was determined. With this extended range of membrane-forming building blocks, this coacervate platform is equipped with tailored permeability through interactions with the coacervate lumen and facilitates sequestration of hydrophobic molecules into the membrane and controlled fluidity.

Abstract Image

控制合成三元共聚物稳定复合共凝胶的膜流动性和生物物理特性
细胞膜是调节细胞内环境组成的重要屏障,可促进对细胞功能和存活至关重要的交流过程。与脂质膜相比,人工聚合物膜通常具有更高的稳定性,因为它们的分子量更高,大分子结构单元的性质也更具可变性,这就为调节流动性和渗透性等重要特性提供了广泛的化学工具箱。我们最近开发了一种基于复合共凝胶的人工细胞平台,其中使用了由亲水性聚乙二醇段、疏水性聚(己内酰胺-三亚甲基碳酸酯)结构域和聚谷氨酸锚(PEG-PCLgTMC-PGA)组成的三元共聚物进行稳定。由于膜的高流动性,这些膜化结构显示出优异的渗透性。然而,事实证明聚合物膜对与环境交换的客体分子的分子量没有选择性。为了推进这一平台的发展,我们合成了一系列具有独特功能的三元共聚物,以进一步完善聚合物膜的调节功能。通过对三元共聚物结构变体(包括疏水结构域基于 PCLgTMC、聚(D,L-乳酸)或聚苯乙烯的变体)的研究,确定了它们对膜渗透性、流动性以及胆固醇等疏水分子螯合作用的影响。有了这些范围更广的成膜构件,这种共凝胶平台就具备了量身定制的特性,有利于将疏水分子封存到膜中并控制流动性。
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来源期刊
Polymer Chemistry
Polymer Chemistry POLYMER SCIENCE-
CiteScore
8.60
自引率
8.70%
发文量
535
审稿时长
1.7 months
期刊介绍: Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.
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