{"title":"In Situ Diagnosis and Digital Cataloguing of Plant Pathogenic Fungi Through Mobile-Based Foldscope Microscopy","authors":"Palanisamy Mooventhan, Palaiyur Nanjappan Sivalingam, Harvinder Kumar Singh, Manoj Kumar Sahu, Yogita Dhimar, Uttam Singh, Pankaj Kaushal, Probir Kumar Ghosh","doi":"10.1111/jph.13422","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Agriculture confronts multifaceted challenges across the spectrum of crop production, with pest and disease management being a prominent concern. Timely diagnosis of crop diseases is imperative for mitigating production costs and curbing the adverse environmental impacts of chemical pesticides. In the present investigation, mobile phone-based foldscope microscopy (MBFM) was used to diagnose various field samples infected with fungal diseases of field and horticultural crops, and the same was validated with the normal microscope pictures and field symptoms. The MBFM was also used to diagnose seed-borne microflora associated with wheat and spores of commercial formulation of bioagents and validated. The MBFM utilises both symptoms and morphological structures of pathogen for in situ field diagnosis and hence advantages over the symptom-based mobile Apps. This study underscores the utility of foldscope microscope as a potent technique for plant pathologists and extension workers to enable real-time and in situ identification of diseases caused by fungal pathogens in various agricultural crops.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"172 6","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13422","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Agriculture confronts multifaceted challenges across the spectrum of crop production, with pest and disease management being a prominent concern. Timely diagnosis of crop diseases is imperative for mitigating production costs and curbing the adverse environmental impacts of chemical pesticides. In the present investigation, mobile phone-based foldscope microscopy (MBFM) was used to diagnose various field samples infected with fungal diseases of field and horticultural crops, and the same was validated with the normal microscope pictures and field symptoms. The MBFM was also used to diagnose seed-borne microflora associated with wheat and spores of commercial formulation of bioagents and validated. The MBFM utilises both symptoms and morphological structures of pathogen for in situ field diagnosis and hence advantages over the symptom-based mobile Apps. This study underscores the utility of foldscope microscope as a potent technique for plant pathologists and extension workers to enable real-time and in situ identification of diseases caused by fungal pathogens in various agricultural crops.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.