pLM33 provides tolerance of persistent Listeria monocytogenes ST5 to various stress conditions and also enhances its virulence

IF 4.5 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology Pub Date : 2024-10-28 DOI:10.1016/j.fm.2024.104675

Xin Liu , Tianqi Shi , Jiaming Li , Huanyu Wu , Qing Zhao , Zhixin Fang , Yingying Liang , Quan Xiao , Min Chen , Qingli Dong , Hongzhi Zhang

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Abstract

Listeria monocytogenes is a major foodborne pathogen. In our previous study, we found that L. monocytogenes ST5 and ST121 strains were dominant in two food plants of Shanghai. Genetic characterization revealed that the environmental tolerance of these strains was attributable to the plasmids pLM33 and pLM5578. To further evaluate the function of L. monocytogenes plasmids, we selected ST5 and ST121 wild-type strains, and used their plasmid-cured strains as controls to conduct tolerance tests. In addition, we analyzed 108 L. monocytogenes strains isolated from four major food categories in Shanghai. Our results showed that compared with the plasmid-cured strain, the ST5 strain carrying pLM33 showed higher tolerance to environmental stress conditions, including low acid, high salt, oxidizing, and high-temperature conditions; as well as higher virulence. Furthermore, we found that the plasmid carriage rate of food isolates was 97.22%, with the highest carriage rate of 68.57% for pLM5578, followed by 24.76% for pLM33. Notably, all L. monocytogenes ST5 isolates from ready-to-eat food products (n = 11) carried plasmids, suggesting that contamination of these food products may pose a serious risk to human health. In summary, the results of this study broaden our understanding regarding the role of L. monocytogenes plasmids in stress responses.

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pLM33 使持久性单核细胞增多性李斯特菌 ST5 能够耐受各种应激条件，并增强其毒力

单核细胞增生李斯特菌是一种主要的食源性致病菌。在之前的研究中，我们发现单核细胞增多性李斯特菌 ST5 和 ST121 菌株在上海的两家食品厂中占优势。遗传特性分析表明，这些菌株对环境的耐受性归因于质粒 pLM33 和 pLM5578。为了进一步评估单核细胞增生性酵母菌质粒的功能，我们选择了 ST5 和 ST121 野生型菌株，并以其质粒固化菌株为对照进行耐受性试验。此外，我们还分析了从上海四大类食品中分离出的 108 株单核细胞增生奈氏菌。结果表明，与质粒固化菌株相比，携带pLM33的ST5菌株对低酸、高盐、氧化和高温等环境胁迫条件的耐受性更高，毒力也更强。此外，我们还发现食物分离株的质粒携带率为 97.22%，其中 pLM5578 的携带率最高，为 68.57%，其次是 pLM33，为 24.76%。值得注意的是，从即食食品（n = 11）中分离出的所有单核细胞增生症 ST5 菌株都携带质粒，这表明这些食品的污染可能对人类健康构成严重威胁。总之，本研究的结果拓宽了我们对单核细胞增生性淋巴瘤质粒在应激反应中的作用的认识。

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来源期刊

Food microbiology 工程技术-生物工程与应用微生物

CiteScore

11.30

自引率

3.80%

发文量

179

审稿时长

44 days

期刊介绍： Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.