Histomorphology based prediction of p16 immunopositivity and p16/HPV DNA co-positivity in oral squamous cell carcinoma

IF 1.5 4区医学 Q3 PATHOLOGY

Annals of Diagnostic Pathology Pub Date : 2024-10-24 DOI:10.1016/j.anndiagpath.2024.152389

Farhat Naz , Nadeem Tanveer , Hitesh Verma , Sudheer Arava , Aanchal Kakkar , Shivam Pandey , Harsh Goel , Amar Ranjan , Anita Chopra , Pranay Tanwar

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引用次数: 0

Abstract

The histomorphological features predictive of p16 and human papilloma virus (HPV DNA) positivity in oropharyngeal carcinoma have been a matter of much debate. However, only few studies have been done on oral squamous cell carcinoma (OSCC) to correlate the histomorphological features with p16 and HPV DNA positivity. Oral squamous cell carcinoma has distinct etiopathogenesis, treatment and prognosis as compared to oropharyngeal carcinomas.

A total of 800 oral squamous cell carcinoma biopsy cases were evaluated for features suggestive of HPV infection like basaloid appearance, absence of stromal reaction, nests and lobules of tumor cells with pushing borders, central necrosis, lympho-epithelial morphology, koilocytes, and non-keratinizing or hybrid morphology. Immunohistochemistry was performed for p16 expression (E6H4 clone, CINtec histology, Roche diagnostics). The cases which showed 2+/3+ (from moderate to high intensity) staining with >75 % cells were considered as p16 immunopositive. All the p16 immunopositive cases were subjected to real-time PCR (polymerase chain reaction) for HPV DNA detection to confirm HPV positivity. A total of 139 (17.37 %) OSCC cases were p16 immunopositive and out of these 104 (104/139, 74.8 %) cases showed HPV-DNA PCR positivity. None of the features were found to be predictive on multivariate logistic regression analysis. However, on bivariable analysis, nest/lobule with pushing border was the only histopathological feature which had a significant correlation with p16 immunopositivity (P value = 0.0001) and p16 and HPV DNA copositivity (P value = 0.0001). (Fisher's exact test -two tailed).

To conclude-morphology is not really predictive of HPV positivity in OSCC cases. Only one feature- nests and lobule with pushing border is suggestive on bivariable analysis.

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基于组织形态学预测口腔鳞状细胞癌的 p16 免疫阳性率和 p16/HPV DNA 共阳性率

口咽癌中可预测 p16 和人乳头状瘤病毒（HPV DNA）阳性的组织形态学特征一直是一个争论不休的问题。然而，只有少数研究对口腔鳞状细胞癌（OSCC）的组织形态特征与 p16 和 HPV DNA 阳性进行了相关性分析。共对 800 例口腔鳞状细胞癌活检病例进行了评估，以确定是否存在提示 HPV 感染的特征，如基底样外观、无基质反应、肿瘤细胞巢状和小叶状且边界推动、中央坏死、淋巴上皮形态、绒毛细胞、非角化或混合形态。免疫组化检测 p16 表达（E6H4 克隆，CINtec 组织学，罗氏诊断公司）。显示 2+/3+（从中度到高度）染色且细胞占 75% 的病例被视为 p16 免疫阳性。对所有 p16 免疫阳性病例进行实时 PCR（聚合酶链反应）HPV DNA 检测，以确认 HPV 阳性。共有 139 例（17.37%）OSCC 病例 p16 免疫阳性，其中 104 例（104/139，74.8%）显示 HPV-DNA PCR 阳性。在多变量逻辑回归分析中，没有发现任何特征具有预测性。然而，在双变量分析中，巢状/小叶状推移边界是唯一与 p16 免疫阳性（P 值 = 0.0001）和 p16 与 HPV DNA 共阳性（P 值 = 0.0001）有显著相关性的组织病理学特征。(总之，形态学并不能真正预测 OSCC 病例中的 HPV 阳性。在双变量分析中，只有一个特征--巢状和边界推移的小叶--具有提示性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Diagnostic Pathology 医学-病理学

CiteScore

3.90

自引率

5.00%

发文量

149

审稿时长

26 days

期刊介绍： A peer-reviewed journal devoted to the publication of articles dealing with traditional morphologic studies using standard diagnostic techniques and stressing clinicopathological correlations and scientific observation of relevance to the daily practice of pathology. Special features include pathologic-radiologic correlations and pathologic-cytologic correlations.