Genome-wide identification and characterization of FORMIN genes in cotton: Implications for abiotic stress tolerance

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2024-10-28 DOI:10.1016/j.plgene.2024.100474

Rasmieh Hamid , Feba Jacob , Zahra Ghorbanzadeh , Mohsen Mardi , Shohreh Ariaeenejad , Mehrshad Zeinalabedini , Mohammad Reza Ghaffari

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引用次数: 0

Abstract

Background

Formins are highly conserved proteins with multiple domains that play an important role in the interaction with microfilaments and microtubules and thus regulate actin organisation and cytoskeletal dynamics. Despite their importance in plant development and response to stress, the study of FORMIN (FH) genes in cotton, an important fibre crop, remains limited. The genetic diversity of these genes is critical for improving the adaptability of cotton to environmental stress, which is a major challenge for cotton breeding programmes aimed at improving abiotic stress tolerance.

Results

Through comprehensive bioinformatics approaches, we identified 46, 50 and 27 putative FH genes in Gossypium hirsutum, G. barbadense and their diploid ancestors G. arboreum and G. raimondii, respectively. A phylogenetic analysis classified these genes into five subfamilies and revealed evolutionary relationships to Arabidopsis thaliana. Syntenic and collinear analyses showed that genomic duplications in cotton have driven the expansion of the FH gene family. Structural analysis showed significant variations in sequence length and conserved motifs. Promoter analysis revealed several cis-acting elements associated with growth, stress response and hormonal signalling. Protein-protein interaction predictions suggest involvement in hormone signalling, cytoskeletal regulation and cell wall dynamics. Differential expression of G. hirsutum FH (GhFH) genes in different cotton tissues under drought and osmotic stress was confirmed by qRT-PCR.

Conclusion

This study provides new insights into the functional diversity and evolutionary dynamics of FH genes in cotton and emphasises their potential role in improving abiotic stress tolerance. By identifying key regulatory genes involved in stress adaptation, this research contributes to the development of more resilient cotton varieties through targeted breeding strategies. The results underline the importance of genetic diversity in enabling cotton breeding programmes to overcome the challenges posed by abiotic stress.

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棉花中 FORMIN 基因的全基因组鉴定和特征描述：对非生物胁迫耐受性的影响

背景Formins是具有多个结构域的高度保守蛋白，在与微丝和微管的相互作用中发挥重要作用，从而调节肌动蛋白的组织和细胞骨架的动态。尽管FORMIN（FH）基因在植物发育和应激反应中具有重要作用，但对棉花这种重要纤维作物中FORMIN（FH）基因的研究仍然有限。结果通过综合生物信息学方法，我们在 Gossypium hirsutum、G. barbadense 及其二倍体祖先 G. arboreum 和 G. raimondii 中分别鉴定出 46、50 和 27 个推测的 FH 基因。系统进化分析将这些基因分为五个亚家族，并揭示了它们与拟南芥的进化关系。同源分析和共线分析表明，棉花基因组的重复推动了 FH 基因家族的扩展。结构分析表明，序列长度和保守基序存在显著差异。启动子分析揭示了几个与生长、应激反应和激素信号有关的顺式作用元件。蛋白质-蛋白质相互作用预测表明，该基因参与激素信号、细胞骨架调节和细胞壁动力学。通过 qRT-PCR 验证了在干旱和渗透胁迫下不同棉花组织中 G. hirsutum FH（GhFH）基因的差异表达。通过确定参与胁迫适应的关键调控基因，这项研究有助于通过有针对性的育种策略培育更具抗逆性的棉花品种。研究结果强调了遗传多样性对于棉花育种计划克服非生物胁迫挑战的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.