Impact of the NH4+/NO3− ratio on growth of oil-rich filamentous microalgae Tribonema minus in simulated nitrogen-rich wastewater

IF 6.3 2区 工程技术 Q1 ENGINEERING, CHEMICAL
Peixia Liang , Huiying Wang , Xinjuan Hu , Mostafa Elshobary , Yi Cui , Bin Zou , Feifei Zhu , Michael Schagerl , Mostafa El-Sheekh , Shuhao Huo
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Abstract

This study delves into the impact of varying NH4+-N: NO3-N ratios in simulated nitrogen-rich wastewater on the physiological and biochemical responses of the Xanthophyceae Tribonema minus. Our findings revealed intriguing patterns: the maximum biomass observed was 5 g/L, which occurred in treatments containing NH4+-N at a concentration of 60 mg/L and NO3-N at a concentration of 180 mg/L (mass concentration ratio of 1:3). Due to the excessive concentration of NH4+-N, the photosynthetic apparatus is damaged. When NH4+-N exceeds 120 mg/L (ratio 1:1 of NH4+-N: NO3-N), growth is seriously hindered. However, increased NH4+-N levels foster lipid accumulation. Notably, NH4+-N removal was high across all NH4+-N treatments, ranging from 78 %–90 %. Nitrate, ammonium, and total phosphorus removal declined with increasing NH4+-N proportions due to limited growth of Tribonema minus. When NH4+-N was 120 mg/L (ratio 1:1 of NH4+-N: NO3-N), the lipid content accounted for 50 % of the biochemical components, surpassing the control group without ammonium addition by 15 %. The lipid composition of T. minus primarily comprises palmitoleic acid, constituting around 50 % of total fatty acids, with its concentration rising at higher NH4+-N supply. Increasing NH4+-N also improved fatty acid profiles and biodiesel properties according to international standards. Overall, this work adds knowledge to stimulate microalgal lipid production. Not only should absolute amounts of nutrients be considered, but also the NH4+-N: NO3-N ratio to optimize biomass yields with high lipid content.

Abstract Image

NH4+/NO3- 比率对模拟富氮废水中富油丝状微藻 Tribonema minus 生长的影响
本研究探讨了模拟富氮废水中不同的 NH4+-N:NO3--N 比值对黄绿藻纲翠贝藻(Tribonema minus)生理生化反应的影响。我们的研究结果揭示了耐人寻味的规律:在NH4+-N浓度为60毫克/升、NO3--N浓度为180毫克/升(质量浓度比为1:3)的处理中,观察到的最大生物量为5克/升。由于 NH4+-N 浓度过高,光合作用装置受到破坏。当 NH4+-N 超过 120 mg/L(NH4+-N:NO3--N 的比例为 1:1)时,生长会受到严重阻碍。然而,NH4+-N 水平的增加会促进脂质积累。值得注意的是,在所有 NH4+-N 处理中,NH4+-N 的去除率都很高,从 78 % 到 90 % 不等。随着 NH4+-N 比例的增加,硝酸盐、铵和总磷的去除率下降,原因是 Tribonema minus 的生长受到限制。当 NH4+-N 为 120 mg/L(NH4+-N:NO3--N 的比例为 1:1)时,脂质含量占生化成分的 50%,比未添加铵的对照组高出 15%。T. minus 的脂质成分主要是棕榈油酸,约占脂肪酸总量的 50%,NH4+-N 供应量越高,其浓度也越高。根据国际标准,增加 NH4+-N 还能改善脂肪酸组成和生物柴油特性。总之,这项研究为促进微藻脂质生产提供了新的知识。不仅要考虑养分的绝对量,还要考虑 NH4+-N:NO3--N 的比例,以优化高脂质含量的生物质产量。
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来源期刊
Journal of water process engineering
Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
10.70
自引率
8.60%
发文量
846
审稿时长
24 days
期刊介绍: The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies
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