Multi-functional PGPR Serratia liquefaciens confers enhanced resistance to lead stress and bacterial blight in soybean (Glycine max L.)

IF 5.4 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2024-10-19 DOI:10.1016/j.cpb.2024.100403

Mohamed A. El-Esawi , Hayssam M. Ali , Ashraf Atef Hatamleh , Munirah Abdullah Al-Dosary , Enas M. El-Ballat

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引用次数: 0

Abstract

Lead toxicity and bacterial blight disease caused by Pseudomonas savastanoi pv. glycinea have destructive impacts on soybean growth and productivity. Plant growth-promoting rhizobacteria have been used as an eco-friendly approach for augmenting crop growth and stress resistance. The current study investigated the efficacy of Serratia liquefaciens ZM6 strain in enhancing soybean resistance to lead (Pb) stress and bacterial blight. Two pot experiments were performed. In the first pot experiment, soybean plants were inoculated with S. liquefaciens ZM6 and grown under variable Pb stress levels (0, 200 and 400 µM of Pb(NO₃)₂). In the second experiment, S. liquefaciens-inoculated soybean plants were infected with P. savastanoi pv. glycinea, and disease severity was assessed two weeks post infection. The results revealed that S. liquefaciens strain resisted Pb stress up to 400 µM Pb(NO₃)₂ and exhibited the highest levels of solubilized phosphate, solubilized zinc, siderophore, indole acetic acid, exopolysaccharide, trehalose and antioxidant enzymes at 400 µM Pb compared to the other treatments. Moreover, Pb stress (200 and 400 µM) significantly decreased the growth, yield, nutrient uptake, gas exchange, and contents of chlorophyll, soluble proteins, sugars, and phenolics of soybean plants. Pb stress also induced the levels of proline, glycine betaine, Pb, oxidative stress markers, antioxidant enzymes, ascorbate, glutathione and expression of stress-responsive genes (CAT, APX, POD, Fe-SOD, CHS7, CHI1A, PAL, IFS2, P5CS and WRKY54) in soybean plants. On the other hand, S. liquefaciens application markedly boosted the growth, yield and levels of nutrients, gas-exchange, chlorophyll, osmolytes, antioxidant enzymes and expression of stress-tolerant genes of Pb-stressed soybean plants. The bacterial inoculation significantly diminished oxidative stress indicators and Pb content in stressed plants. Inoculation of soybean plants with S. liquefaciens also caused significant reductions in blight disease symptoms in P. savastanoi pv. glycinea-infected plants, indicating the efficiency of this strain in controlling harmful blight disease. Overall, this study demonstrated S. liquefaciens ZM6 effectiveness in enhancing soybean resistance to Pb stress and bacterial blight.

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多功能 PGPR Serratia liquefaciens 增强了大豆（Glycine max L.）对铅胁迫和细菌性枯萎病的抗性

铅毒性和由 Pseudomonas savastanoi pv. glycinea 引起的细菌性枯萎病对大豆的生长和产量具有破坏性影响。植物生长促进根瘤菌已被用作提高作物生长和抗逆性的生态友好型方法。本研究调查了 Serratia liquefaciens ZM6 菌株在增强大豆对铅（Pb）胁迫和细菌性枯萎病的抗性方面的功效。研究进行了两次盆栽实验。在第一个盆栽实验中，大豆植株接种了 S. liquefaciens ZM6，并在不同的铅胁迫水平（0、200 和 400 µM 的 Pb(NO3)2）下生长。在第二个实验中，接种了 S. liquefaciens 的大豆植株感染了 P. savastanoi pv. glycinea，并在感染两周后评估了病害严重程度。结果表明，与其他处理相比，S. liquefaciens 菌株能抵抗高达 400 µM Pb(NO3)2 的铅胁迫，并在 400 µM Pb 时表现出最高水平的溶解磷酸盐、溶解锌、苷元、吲哚乙酸、外多糖、三卤糖和抗氧化酶。此外，铅胁迫（200 和 400 µM）显著降低了大豆植株的生长、产量、养分吸收、气体交换以及叶绿素、可溶性蛋白质、糖和酚的含量。铅胁迫还诱导大豆植株中脯氨酸、甘氨酸甜菜碱、铅、氧化胁迫标记物、抗氧化酶、抗坏血酸、谷胱甘肽的水平以及胁迫响应基因（CAT、APX、POD、Fe-SOD、CHS7、CHI1A、PAL、IFS2、P5CS 和 WRKY54）的表达。另一方面，液化大豆酵母菌的应用明显促进了铅胁迫大豆植株的生长、产量和养分、气体交换、叶绿素、渗透溶质、抗氧化酶水平以及抗胁迫基因的表达。接种细菌后，受胁迫植物体内的氧化胁迫指标和铅含量明显降低。给大豆植株接种 S. liquefaciens 还能显著减轻 P. savastanoi pv. glycinea 感染植株的枯萎病症状，这表明该菌株能有效控制有害的枯萎病。总之，这项研究证明了 S. liquefaciens ZM6 在增强大豆对铅胁迫和细菌性枯萎病的抗性方面的有效性。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.