Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-10-31 DOI:10.1016/j.sajb.2024.10.053

Yi-Ju Chen , Ching-Fang Chang , Rathinasamy Baskaran , Chih-Hung Liang , Hsin-Ning Kuo , Mosleh Mohammad Abomughaid , Wan-Teng Lin

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引用次数: 0

Abstract

Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis. 9% potato protein (PP) hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments. PPH902 at 1600 ppm does not affect the cell viability of 3T3-L1 adipocytes. PPH902 regulates the expression of lipid production-related transcription factors PPARγ, SREBP-1c, and FAS by activating AMPK, thereby inhibiting lipogenesis, and activating phosphorylated HSL to promote lipolysis to increase lipid metabolism. These results show that PPH902 is an effective anti-lipogenesis and lipolysis-promoting hydrolyzate, which has the potential to develop anti-obesity products.

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在脂肪细胞中处理马铃薯蛋白水解物（PPH902）可通过激活 AMPK 抑制脂肪生成

肥胖症是由热量摄入不均衡引起的慢性代谢性疾病，对人体健康危害严重，增加了高血压、高脂血症、糖尿病和心血管疾病的发病风险。以往的研究表明，经钙化酶处理衍生的马铃薯蛋白水解物（PPH）在动物实验中具有调节血压和血糖的作用，并具有保护心脏、肝脏和抑制肌肉蛋白降解的活性。本研究旨在筛选最佳条件，通过调节脂肪生成和脂肪分解机制，分离出具有潜在抗肥胖活性的马铃薯蛋白水解物。水解2小时的9%马铃薯蛋白（PP）（PPH902）产量高，活性更好，因此在所有其他实验中都使用了PPH902。百万分之 1600 的 PPH902 不会影响 3T3-L1 脂肪细胞的活力。PPH902 通过激活 AMPK 从而抑制脂肪生成，并激活磷酸化 HSL 促进脂肪分解以增加脂质代谢，从而调节与脂质生成相关的转录因子 PPARγ、SREBP-1c 和 FAS 的表达。这些结果表明，PPH902 是一种有效的抗脂肪生成和促进脂肪分解的水解物，具有开发抗肥胖产品的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464