Mating modifies oxidative stress in the brain and confers protection against Parkinson's Disease in a Drosophila model

Abstract

Mating exerts profound and multifaceted effects on the physiology of female insects, particularly influencing metabolic alterations and bolstering stress resilience. Drosophila melanogaster has emerged as an excellent model to investigate the mechanism of neurodegenerative diseases. However, interplay between mating and its impact on the Drosophila brain remains a tantalizing enigma, awaiting elucidation. Herein, we reported that mating significantly improved the climbing and jumping activity in mated females compared to the virgins in Drosophila. Mating also reduced oxidative stress in the brain. Based on the results, we found that, mated females exhibited better behavioral performance and fewer loss of dopaminergic (DA) neurons than unmated females in PINK1 RNAi flies, a well-established Parkinson's disease (PD) model. Further study demonstrated that mating led to decreased iron content in the brain, a process associated with decreased Transferrin 1 (Tsf1) and Malvolio (Mvl) and increased ferritin. Additionally, mating inhibited expression of Duox and Nox, two NADPH oxidases in Drosophila. Furthermore, Kr-h1, a transcription factor of JH, acted downstream of mating to regulate genes involved in iron metabolism and NADPH oxidases. Collectively, the findings suggested a pivotal role of mating in regulating iron metabolism and NADPH oxidases in the brain of Drosophila. Consequently, considering mating status is imperative in scientific research, particularly in the context of neurological disorders.