Morpho-biochemical and molecular characterization of new mung bean [Vigna radiata (L.) Wilczek] landraces for Cercospora leaf spot (CLS) disease resistance

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2024-11-01 DOI:10.1016/j.pmpp.2024.102448

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引用次数: 0

Abstract

Mung bean production is significantly lowered by Cercospora leaf spot (CLS) in various parts of the globe. The most effective way to increase mung bean resilience to this stress is to identify new CLS resistance sources. Therefore, a panel population of 13 landraces along with 2 susceptible check cultivar, and 11 SSR markers were taken for the present experiment. Correlation analysis suggested significant relationships between the quantitative traits, retained resistance (Banapur local, and Kalampur local), and moderately resistance (Dikhitapada local, and Fakirpur local 1) to CLS. However, total chlorophyll content (TCL) showed strong positive correlations with phenolic content (PHE) (0.984), peroxidase activity (POX) (0.931), and polyphenol oxidase activity (PPO) (0.969). Moreover, PHE (−0.769) and POX (−0.867) were also found negatively correlated with CLS disease reaction. The eigenvalues from the principal component analysis (PCA) indicated the first three principal components collectively explained 84.88 % of the total variance. Molecular analysis of the panel population suggested a PIC value range from 0.30 to 0.88, with an average 0.696, and markers such as cp01713 (0.84), cp02181 (0.87), and CEDG147 (0.88) had higher PIC values. Principal coordinate analysis (PCoA) suggested capturing of 69.06 % of the total variance by the first three axes, and the landraces were categorizing into three clusters (cluster A, B and C). The genetic distances among the landraces ranged from 0 to 0.608, and phylogenetic tree differentiated them into three major clusters at respective Jaccard's similarity coefficient i.e., Cluster I (0.68), II (0.75) and III (0.83). Meanwhile, the resistance and moderately resistance landraces were observed in cluster A and cluster B, and in cluster II and cluster III respectively. The resistance landraces obtained from the present study could be used as potential donor for breeding process towards CLS resistance in mung bean.

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新绿豆[Vigna radiata (L.) Wilczek]陆生品系抗Cercospora叶斑病(CLS)的形态生化和分子特性鉴定

在全球不同地区，绿豆的产量因Cercospora叶斑病（CLS）而大幅下降。提高绿豆抗逆性的最有效方法是鉴定新的 CLS 抗性来源。因此，本实验选取了 13 个地方品种和 2 个易感对照栽培品种，以及 11 个 SSR 标记。相关分析表明，数量性状、对 CLS 的保留抗性（Banapur 地方品种和 Kalampur 地方品种）和中度抗性（Dikhitapada 地方品种和 Fakirpur 地方品种 1）之间存在显著的相关性。然而，总叶绿素含量（TCL）与酚含量（PHE）（0.984）、过氧化物酶活性（POX）（0.931）和多酚氧化酶活性（PPO）（0.969）呈强正相关。此外，还发现 PHE（-0.769）和 POX（-0.867）与 CLS 疾病反应呈负相关。主成分分析（PCA）的特征值表明，前三个主成分共同解释了总方差的 84.88%。小组群体的分子分析表明，PIC 值范围在 0.30 至 0.88 之间，平均为 0.696，cp01713（0.84）、cp02181（0.87）和 CEDG147（0.88）等标记的 PIC 值较高。主坐标分析（PCoA）结果表明，前三个坐标轴占据了总方差的 69.06%，并将陆稻品种划分为三个群（群 A、群 B 和群 C）。各地方品种之间的遗传距离在 0 至 0.608 之间，系统发育树以各自的 Jaccard 相似系数将它们区分为三个主要群，即群组 I（0.68）、群组 II（0.75）和群组 III（0.83）。同时，抗性和中抗性陆稻分别出现在簇 A 和簇 B 中，以及簇 II 和簇 III 中。本研究获得的抗性地方品种可作为绿豆抗 CLS 育种的潜在供体。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.