Physical-Chemical Characterisation and Biological Activities of the Essential Oil of Lippia alba (Mill) NE Br Ex Britton Obtained in Ecuador

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2024-10-05 DOI:10.1016/j.hermed.2024.100951

C. Tubay-Bermùdez , C.A. Neves , A.A. Dueñas-Rivadeneira , A.M. Peña , L.A.Z. Mendoza , K.R. Escobar , D.M. Rivas-Navia , N.R. Maddela

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引用次数: 0

Abstract

Introduction

Lippia alba is a wild semishrubby species that grows in countries like Ecuador, its extracts have significant bioactive compounds. However, the antioxidant and antimicrobial properties of L alba essential oil (EO) have not been given importance in this region. The present study aimed to evaluate the physicochemical characteristics of the extracts and biological activities of L alba EO.

Methods

A unifactorial design was used, and physicochemical variables (pH, density, and refractive index) and antioxidant and antimicrobial capacities were explored using standard methods. Fractions of L alba EO were analysed by gas chromatography-mass spectroscopy.

Results

The density, pH, refractive index, and phenolic content of L alba EO were found to be 0.95 g/ml, 5.5, 1.51, and 918 mg/l, respectively. An antioxidant activity of 23.08 mg/l was observed by the 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic) method, while 14.81 mg/l was observed by the ferric reducing antioxidant power method. Essential oil of L alba has presented a greater antimicrobial activity against different Gram-negative than Gram-positive bacteria. Essential oil of L alba showed an inhibitory zone of 56.7 and 9.7 mm against Photobacterium damselae sub sp. piscicida DSM 22834 and Bacillus subtilis ATCC 6633, respectively.

Conclusions

The results of the present investigation clearly indicated that the EO of L alba has potential antioxidant and antimicrobial activities in vitro analysis, which are important indications that this extract can be applied in different industries, such as pharmaceutical industry, food industry, and others.

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厄瓜多尔出产的 Lippia alba（Mill）NE Britton Ex Britton 精油的物理化学特征和生物活性

导言：白杓兰是一种生长在厄瓜多尔等国家的野生半灌木物种，其提取物具有重要的生物活性化合物。然而，白柠檬精油（EO）的抗氧化和抗菌特性在该地区尚未得到重视。本研究旨在评估白叶桉树精油提取物的理化特性和生物活性。研究方法采用单因素设计，使用标准方法探讨理化变量（pH 值、密度和折射率）以及抗氧化和抗菌能力。结果发现白叶桉油的密度、pH 值、折射率和酚含量分别为 0.95 克/毫升、5.5、1.51 和 918 毫克/升。2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic) 法观察到的抗氧化活性为 23.08 毫克/升，铁还原抗氧化能力法观察到的抗氧化活性为 14.81 毫克/升。白叶鳞茎精油对不同革兰氏阴性菌的抗菌活性高于革兰氏阳性菌。本研究的结果清楚地表明，白叶桉树精油在体外分析中具有潜在的抗氧化和抗菌活性，这是该提取物可应用于制药业、食品业等不同行业的重要标志。

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.