Ethyl esters synthesis catalyzed by lipase B from Candida antarctica immobilized on NiFe2O4 magnetic nanoparticles

IF 5.2 2区化学 Q1 CHEMISTRY, APPLIED

Catalysis Today Pub Date : 2024-10-18 DOI:10.1016/j.cattod.2024.115099

Gabrielle A.R. da Silva , Thamires M. de L.O. da Silva , João Paulo da S.Q. Menezes , Elizabeth Cristina T. Veloso , Gizele C. Fontes-Sant’Ana , Noemi Raquel C. Huaman , Rodrigo Brackmann , Marta A.P. Langone

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Abstract

The development of heterogeneous biocatalysts allows the expansion of the application of enzymes in different industrial processes, favoring the establishment of clean technologies. This work investigates the capacity of nickel ferrite magnetic nanoparticles (NiFe₂O₄) as a support for lipase B from Candida antarctica (CalB) immobilization. The adsorption capacity of the support revealed a maximum value of 15 mg_protein/g_support, according to the Langmuir isotherm model. Efficiency immobilization by physical adsorption was low (21.3 %), and CalB was covalently immobilized after functionalization of NiFe₂O₄ with APTMS (3-aminopropyl trimethoxysilane) and activation with glutaraldehyde (GA), showing higher immobilization yield (62.9 %). The spinel ferrite NiFe₂O₄ was characterized by many physicochemical analyses. The enzyme derivatives obtained (NiFe₂O₄-CalB and NiFe₂O₄-APTMS-GA-CalB) were evaluated in the synthesis of alkyl esters. Despite no production was observed in the transesterification reactions, esterification led to 37.3 ± 1.0 % and 62.1 ± 0.2 % of oleic acid conversions using NiFe₂O₄-APTMS-GA-CalB and NiFe₂O₄-CalB, respectively. After 4 cycles, NiFe₂O₄-CalB maintained 92 % of its initial activity. Nickel ferrite magnetic nanoparticles form an efficient heterogeneous catalyst with lipase, which could be used to remove the content of free fatty acids (FFAs), like oleic acid, present in cheap raw materials to produce biodiesel.

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固定在 NiFe2O4 磁性纳米粒子上的白色念珠菌脂肪酶 B 催化的乙酯合成

异质生物催化剂的开发扩大了酶在不同工业流程中的应用，有利于建立清洁技术。这项工作研究了镍铁氧体磁性纳米颗粒（NiFe2O4）作为一种支持物对来自南极念珠菌（CalB）的脂肪酶 B 的固定能力。根据 Langmuir 等温线模型，吸附剂的最大吸附容量为 15 mg 蛋白/gsupport。物理吸附的固定化效率较低（21.3%），而用 APTMS（3-氨基丙基三甲氧基硅烷）对 NiFe2O4 进行功能化并用戊二醛（GA）活化后，CalB 被共价固定化，固定化率较高（62.9%）。对尖晶石铁氧体 NiFe2O4 进行了多种物理化学分析。获得的酶衍生物（NiFe2O4-CalB 和 NiFe2O4-APTMS-GA-CalB）在合成烷基酯时进行了评估。尽管在酯交换反应中没有观察到生成，但使用 NiFe2O4-APTMS-GA-CalB 和 NiFe2O4-CalB 进行酯交换时，油酸的转化率分别为 37.3 ± 1.0 % 和 62.1 ± 0.2 %。经过 4 个循环后，NiFe2O4-CalB 保持了其初始活性的 92%。镍铁氧体磁性纳米粒子与脂肪酶形成了一种高效的异相催化剂，可用于去除廉价原料中的游离脂肪酸（FFA）含量，如油酸，以生产生物柴油。

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来源期刊

Catalysis Today 化学-工程：化工

CiteScore

11.50

自引率

3.80%

发文量

573

审稿时长

2.9 months

期刊介绍： Catalysis Today focuses on the rapid publication of original invited papers devoted to currently important topics in catalysis and related subjects. The journal only publishes special issues (Proposing a Catalysis Today Special Issue), each of which is supervised by Guest Editors who recruit individual papers and oversee the peer review process. Catalysis Today offers researchers in the field of catalysis in-depth overviews of topical issues. Both fundamental and applied aspects of catalysis are covered. Subjects such as catalysis of immobilized organometallic and biocatalytic systems are welcome. Subjects related to catalysis such as experimental techniques, adsorption, process technology, synthesis, in situ characterization, computational, theoretical modeling, imaging and others are included if there is a clear relationship to catalysis.