Chitosan/graphene oxide based biocomposite dynamic films for enzyme-free biosensing application

IF 3.9 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials Science and Engineering B-advanced Functional Solid-state Materials Pub Date : 2024-10-30 DOI:10.1016/j.mseb.2024.117766

Muhammad Usama , Musammir Khan , Xingzhou Peng , Junjie Wang

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Abstract

Biocomposite films of chitosan (CS) and graphene oxide (GO) filler using glutaraldehyde crosslinker was prepared by Schiff-base linkages for enzyme-free biosensing applications. The obtained biocomposites (CS/GO) was characterized by different physicochemical techniques. The glassy carbon electrode (GCE) modified with these biocomposites indicated enhanced redox peak currents and peak separation potentials, as well as accompanied by a reduction in electron transfer resistance as compared with pristine CS material. The CS/GO modified GCE was tested for biosensing in the linear concentration range of glucose (Gl) ∼ 1.25 to 125 ppm, gallic acid (GA) ∼ 6.25 to 75 ppm and dopamine (DA) ∼ 25 to 100 ppm. Moreover, the biosensor indicated high sensitivity (72.9 µA.mM⁻¹.cm⁻²), lower detection limit (0.094 mM) and lower quantification limit (0.313 mM) against Gl as compared with previous reported values. The proposed electro-oxidation mechanism on the modified GCE surface indicated the biocomposites as promising green electroactive smart materials for enzyme-free biosensing applications.

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基于壳聚糖/氧化石墨烯的生物复合动态薄膜在无酶生物传感中的应用

利用戊二醛交联剂，通过席夫碱连接制备了壳聚糖（CS）和氧化石墨烯（GO）填料的生物复合薄膜，用于无酶生物传感应用。获得的生物复合材料（CS/GO）通过不同的理化技术进行了表征。与原始 CS 材料相比，用这些生物复合材料修饰的玻璃碳电极（GCE）的氧化还原峰电流和峰值分离电位均有所提高，同时电子转移电阻也有所降低。经 CS/GO 修饰的 GCE 在葡萄糖（Gl）± 1.25 至 125 ppm、没食子酸（GA）± 6.25 至 75 ppm 和多巴胺（DA）± 25 至 100 ppm 的线性浓度范围内进行了生物传感测试。此外，与之前报道的数值相比，该生物传感器对 Gl 的灵敏度高（72.9 µA.mM-1.cm-2），检测限低（0.094 mM），定量限低（0.313 mM）。所提出的修饰 GCE 表面的电氧化机制表明，生物复合材料是一种很有前途的绿色电活性智能材料，可用于无酶生物传感应用。

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来源期刊

Materials Science and Engineering B-advanced Functional Solid-state Materials 工程技术-材料科学：综合

CiteScore

5.60

自引率

2.80%

发文量

481

审稿时长

3.5 months

期刊介绍： The journal provides an international medium for the publication of theoretical and experimental studies and reviews related to the electronic, electrochemical, ionic, magnetic, optical, and biosensing properties of solid state materials in bulk, thin film and particulate forms. Papers dealing with synthesis, processing, characterization, structure, physical properties and computational aspects of nano-crystalline, crystalline, amorphous and glassy forms of ceramics, semiconductors, layered insertion compounds, low-dimensional compounds and systems, fast-ion conductors, polymers and dielectrics are viewed as suitable for publication. Articles focused on nano-structured aspects of these advanced solid-state materials will also be considered suitable.