Increased inflammation in older high-pressure glaucoma mice

IF 3.7 3区医学 Q2 GERIATRICS & GERONTOLOGY

Neurobiology of Aging Pub Date : 2024-10-09 DOI:10.1016/j.neurobiolaging.2024.10.001

Sabrina Reinehr , M. Rahim Pamuk , Rudolf Fuchshofer , H. Burkhard Dick , Stephanie C. Joachim

{"title":"Increased inflammation in older high-pressure glaucoma mice","authors":"Sabrina Reinehr , M. Rahim Pamuk , Rudolf Fuchshofer , H. Burkhard Dick , Stephanie C. Joachim","doi":"10.1016/j.neurobiolaging.2024.10.001","DOIUrl":null,"url":null,"abstract":"<div><div>Besides an elevated intraocular pressure (IOP), advanced age is one of the most crucial risk factors for developing glaucoma. βB1-Connective Tissue Growth Factor (βB1-CTGF) high-pressure glaucoma mice were used in this study to assess whether glaucoma mice display more inflammatory and aging processes than age-matched controls. Therefore, 20-month-old βB1-CTGF and corresponding wildtype (WT) controls were examined. After IOP measurements, retinas were processed for (immuno-)histological and quantitative real-time PCR analyses. A significantly higher IOP and diminished retinal ganglion cell numbers were noted in βB1-CTGF mice compared to WT. An enhanced macrogliosis as well as an increased number of microglia/macrophages and microglia was detected in retinas of old glaucoma mice. Interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and transforming growth factor-β2 were upregulated, suggesting an ongoing inflammation. Moreover, βB1-CTGF retinas displayed an increased senescence-associated β-galactosidase staining accompanied by a downregulation of <em>Lmnb1</em> (laminin-B1) mRNA levels. Our results provide a deeper insight into the association between inflammation and high-pressure glaucoma and thus might help to develop new therapy strategies.</div></div>","PeriodicalId":19110,"journal":{"name":"Neurobiology of Aging","volume":"145 ","pages":"Pages 55-64"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurobiology of Aging","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0197458024001702","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GERIATRICS & GERONTOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Besides an elevated intraocular pressure (IOP), advanced age is one of the most crucial risk factors for developing glaucoma. βB1-Connective Tissue Growth Factor (βB1-CTGF) high-pressure glaucoma mice were used in this study to assess whether glaucoma mice display more inflammatory and aging processes than age-matched controls. Therefore, 20-month-old βB1-CTGF and corresponding wildtype (WT) controls were examined. After IOP measurements, retinas were processed for (immuno-)histological and quantitative real-time PCR analyses. A significantly higher IOP and diminished retinal ganglion cell numbers were noted in βB1-CTGF mice compared to WT. An enhanced macrogliosis as well as an increased number of microglia/macrophages and microglia was detected in retinas of old glaucoma mice. Interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and transforming growth factor-β2 were upregulated, suggesting an ongoing inflammation. Moreover, βB1-CTGF retinas displayed an increased senescence-associated β-galactosidase staining accompanied by a downregulation of Lmnb1 (laminin-B1) mRNA levels. Our results provide a deeper insight into the association between inflammation and high-pressure glaucoma and thus might help to develop new therapy strategies.

查看原文本刊更多论文

老年高压青光眼小鼠炎症加剧

除了眼内压（IOP）升高外，高龄也是罹患青光眼的最关键风险因素之一。本研究利用βB1-结缔组织生长因子（βB1-CTGF）高压青光眼小鼠来评估青光眼小鼠是否比年龄匹配的对照组表现出更多的炎症和衰老过程。因此，研究人员对 20 个月大的βB1-CTGF 小鼠和相应的野生型（WT）对照组进行了检查。测量眼压后，对视网膜进行（免疫）组织学和实时定量 PCR 分析。与 WT 相比，βB1-CTGF 小鼠的眼压明显升高，视网膜神经节细胞数量减少。在老年性青光眼小鼠的视网膜中，检测到大胶质细胞增多以及小胶质细胞/巨噬细胞和小胶质细胞数量增加。白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α和转化生长因子-β2上调，表明炎症正在持续。此外，βB1-CTGF 视网膜显示衰老相关的 β-半乳糖苷酶染色增加，同时 Lmnb1（层粘连蛋白-B1）mRNA 水平下调。我们的研究结果让我们更深入地了解了炎症与高压性青光眼之间的关联，从而可能有助于开发新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Neurobiology of Aging 医学-老年医学

CiteScore

8.40

自引率

2.40%

发文量

225

审稿时长

67 days

期刊介绍： Neurobiology of Aging publishes the results of studies in behavior, biochemistry, cell biology, endocrinology, molecular biology, morphology, neurology, neuropathology, pharmacology, physiology and protein chemistry in which the primary emphasis involves mechanisms of nervous system changes with age or diseases associated with age. Reviews and primary research articles are included, occasionally accompanied by open peer commentary. Letters to the Editor and brief communications are also acceptable. Brief reports of highly time-sensitive material are usually treated as rapid communications in which case editorial review is completed within six weeks and publication scheduled for the next available issue.