Trolox Reduces Neuroblastoma-Induced Oxidative Stress and Inflammation.

Turkish neurosurgery Pub Date : 2024-01-01 DOI:10.5137/1019-5149.JTN.46442-24.4

Haydar Celik, Guven Akcay, Aysenur Budak Savas, Fatma Yesilyurt, Filiz Demirdogen, Dilanur Ates, Ahmet Hacimuftuoglu

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Abstract

Aim: To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line.

Material and methods: SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 μM and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed.

Results: Administration of 200 μM of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 μM of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results.

Conclusion: The results of this experimental study demonstrated a Trolox concentration of 200 μM produces an anticancer effect on NB cell-line.

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Trolox 可降低神经母细胞瘤细胞系诱导的氧化应激和炎症。

目的：众所周知，Trolox 是过氧自由基和烷氧基自由基的直接清除剂，具有抗氧化作用。神经母细胞瘤（NB）是儿童早期颅外实体瘤中的第二大肿瘤，约三分之二的病例发生在 5 岁之前，约占儿童癌症死亡总数的 15%。由于对 NB 的治疗不足，导致高危患者的存活率很低。本研究旨在确定三氯氧磷抗癌剂量对神经母细胞瘤癌系（SH-SY5Y 细胞系）细胞毒性、氧化剂、抗氧化剂和细胞因子水平的影响：在细胞培养中，SH-SY5Y 细胞在适当的营养培养基和条件下生长。将五种不同剂量的 Trolox（50、100、200、400 和 800 μM）应用于细胞系 24 和 48 小时。通过 MTT 细胞毒性测试、总氧化剂和总抗氧化剂测试以及促炎细胞因子测试对三氯杀螨醇的应用进行了分析：结果：根据获得的数据，应用 200 μM Trolox 可使癌细胞存活率降低达 77.76%。三氯氧烷对 SH-SY5Y 细胞系的影响呈剂量依赖性。此外，200 μM Trolox 还能降低神经母细胞瘤细胞的氧化活性，提高抗氧化能力，降低促炎细胞因子水平，这与细胞毒性测试结果一致：结果表明，200 μM Trolox 对神经母细胞瘤细胞株有抗癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Turkish neurosurgery

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