Boosting bepotastine fluorescence by switching off intramolecular photoinduced electron transfer: Application to eye drops and aqueous humor

IF 4.3 2区 化学 Q1 SPECTROSCOPY
Aya Magdy Saad , Jenny Jeehan Mohamed Nasr , Asmaa Kamal El-Deen
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Abstract

Bepotastine (BPT), a second-generation antihistamine used for treating allergic rhinitis and pruritus, requires precise dosage calculation and accurate measurement in aqueous humor to ensure therapeutic efficacy and avoid adverse effects. This study presents a highly sensitive, cost-effective, readily-implementable, and ecologically sustainable method to detect BPT by enhancing its weak native fluorescence. This has been achieved by switching off the intramolecular photoinduced electron transfer (PET) process through phosphoric acid-induced protonation of the nitrogen in the piperidinyl ring. Further fluorescence enhancement was achieved by incorporating a solution of sodium dodecyl sulfate (SDS) surfactant thus increasing the quantum yield of BPT up to 10 folds. Fluorescence intensity was measured at 425 nm following excitation at 265 nm. The method, validated according to ICH guidelines, demonstrated linearity over the range of 0.70–6.0 µg/mL with a correlation coefficient of 0.9972 and a low detection limit of 0.046 µg/mL. It was successfully applied to measure BPT in aqueous humor and eye drops with % recoveries between 97.59 % and 102.62 %. Finally, sustainability was assessed using various tools, confirming the method’s practicality for routine clinical and pharmaceutical quality control analyses.

Abstract Image

通过关闭分子内光诱导电子转移来增强贝泊他丁的荧光:应用于滴眼液和眼房水。
贝泊他汀(BPT)是一种用于治疗过敏性鼻炎和瘙痒症的第二代抗组胺药,需要精确计算剂量并准确测量水样中的含量,以确保疗效并避免不良反应。本研究提出了一种高灵敏度、高成本效益、可随时实施且生态上可持续的方法,通过增强 BPT 的微弱原生荧光来检测 BPT。这是通过磷酸诱导哌啶环中氮的质子化来关闭分子内光诱导电子转移(PET)过程实现的。通过加入十二烷基硫酸钠(SDS)表面活性剂溶液,进一步增强了荧光,从而将 BPT 的量子产率提高了 10 倍。在 265 nm 处激发后,在 425 nm 处测量荧光强度。该方法在 0.70-6.0 µg/mL 范围内线性关系良好,相关系数为 0.9972,检出限低至 0.046 µg/mL。该方法被成功地用于测量水溶液和滴眼液中的 BPT,回收率在 97.59 % 到 102.62 % 之间。最后,使用各种工具对该方法的可持续性进行了评估,证实了该方法在常规临床和药品质量控制分析中的实用性。
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来源期刊
CiteScore
8.40
自引率
11.40%
发文量
1364
审稿时长
40 days
期刊介绍: Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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