Melatonin associated with bacterial cellulose-based hydrogel improves healing of skin wounds in diabetic rats.

Acta cirurgica brasileira Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI:10.1590/acb399024

Jaiurte Gomes Martins da Silva, Ismaela Maria Ferreira de Melo, Érique Ricardo Alves, Glícia Maria de Oliveira, Anderson Arnaldo da Silva, Flávia Cristina Morone Pinto, José Lamartine de Andrade Aguiar, Diego Neves Araújo, Valéria Wanderley Teixeira, Álvaro Aguiar Coelho Teixeira

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Abstract

Purpose: To describe the effects of melatonin associated with bacterial cellulose-based hydrogel on healing of skin wounds in diabetic rats.

Methods: Streptozotocin was used to induce diabetes in Wistar rats. After wound induction, animals were randomly divided into groups GC, GDCC, GDCB, and GDMCB. Animals were evaluated in days 3, 7, and 14 for the following variables: glycemic levels, histopathological and histochemical analyses, healing rate, morphometry and C-reactive protein.

Results: There was no change in glycemic levels in the diabetic animals as a result of the treatments; histopathological analyses showed better healing in GDCB and GDMCB groups, as well as histochemistry; at day 14, the highest healing rate was observed in animals from the GDMCB group, reaching almost 100%; morphometry revealed a significant increase of fibroblasts and reduction of macrophages and blood vessels in lesions treated with bacterial cellulose associated or not with melatonin when compared to the other experimental groups. There was also an increase in C-reactive protein in GDCC group at day 14.

Conclusion: Bacterial cellulose-based dressings associated with systemic melatonin showed beneficial results in experimentally induced wounds in diabetic rats, favoring the healing process.

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褪黑素与细菌纤维素水凝胶可改善糖尿病大鼠皮肤伤口的愈合。

目的：描述褪黑素与细菌纤维素水凝胶对糖尿病大鼠皮肤伤口愈合的影响：方法：使用链脲佐菌素诱导 Wistar 大鼠患糖尿病。伤口诱导后，动物被随机分为 GC 组、GDCC 组、GDCB 组和 GDMCB 组。在第 3、7 和 14 天对动物进行以下评估：血糖水平、组织病理学和组织化学分析、愈合率、形态测量和 C 反应蛋白：结果：治疗后，糖尿病动物的血糖水平没有变化；组织病理学分析表明，GDCB 组和 GDMCB 组的愈合效果更好，组织化学分析也是如此；第 14 天，GDMCB 组动物的愈合率最高，几乎达到 100%；形态测量显示，与其他实验组相比，使用或未使用褪黑素的细菌纤维素治疗的病变组织中，成纤维细胞明显增加，巨噬细胞和血管减少。第 14 天时，GDCC 组的 C 反应蛋白也有所增加：基于细菌纤维素的敷料与全身性褪黑素联合使用，对糖尿病大鼠的实验性伤口有好处，有利于伤口愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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