Virulence genes and antibiotic susceptibility patterns of Escherichia coli isolated from nosocomial urinary tract infections in the northwest of Iran during 2022–2023: A cross-sectional study

IF 2.1 Q2 MEDICINE, GENERAL & INTERNAL

Health Science Reports Pub Date : 2024-10-30 DOI:10.1002/hsr2.70149

Mohammad Yousef Memar, Masoud Vosughi, Yalda Rahbar Saadat, Mohammadreza Ardalan, Mina Yekani, Bahram Niknafs, Sepideh Zununi Vahed

{"title":"Virulence genes and antibiotic susceptibility patterns of Escherichia coli isolated from nosocomial urinary tract infections in the northwest of Iran during 2022–2023: A cross-sectional study","authors":"Mohammad Yousef Memar, Masoud Vosughi, Yalda Rahbar Saadat, Mohammadreza Ardalan, Mina Yekani, Bahram Niknafs, Sepideh Zununi Vahed","doi":"10.1002/hsr2.70149","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background and Aims</h3>\n \n <p>Urinary tract infections (UTIs) are prevalent among hospitalized patients, constituting the most frequent health-care infections. Uropathogenic <i>Escherichia coli</i> (UPEC) is leading causative agent of UTIs. The present study was aimed to examine the susceptibility of UPEC isolates obtained from nosocomial cases to antibiotics, as well as their biofilm formation capability and frequency of virulence genes.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A total of 100 UPEC isolates were collected from nosocomial UTIs at Imam Reza Hospitals in Tabriz, Iran, spanning from April 2022 to January 2023. The antimicrobial susceptibility patterns were evaluated using the disk diffusion method, along with the detection of broad-spectrum β-lactam enzymes (ESBLs) and carbapenemases. The ability of isolates to form biofilms was assessed using the microtiter-plate method, while the PCR method was employed to identify the presence of virulence genes.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The highest resistance was observed toward piperacillin (82%), followed by aztreonam and ciprofloxacin (81%), while the lowest resistance was found against piperacillin/tazobactam (12%) and meropenem (9%). ESBLs were detected in 62% of the isolates. The microtiter-plate results revealed strong, moderate, and weak biofilm formation abilities in 32%, 33%, and 24% of the isolates, respectively. The most prevalent virulence gene was <i>fimA</i> (74%) followed by <i>hlyF</i> (68%), <i>papA</i> (44%), <i>papC</i> (32%), <i>iroN</i> (26%), and <i>cnf</i> (20%).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The elevated levels of resistance to multiple antimicrobial agents, coupled with the co-presence of virulence genes and biofilm formation abilities, contribute to the persistence of UPEC-related infections, particularly in hospitalized patients. These findings underscore the necessity of implementing an effective program to control nosocomial UTIs caused by UPEC in the healthcare centers.</p>\n </section>\n </div>","PeriodicalId":36518,"journal":{"name":"Health Science Reports","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522605/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Health Science Reports","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/hsr2.70149","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background and Aims

Urinary tract infections (UTIs) are prevalent among hospitalized patients, constituting the most frequent health-care infections. Uropathogenic Escherichia coli (UPEC) is leading causative agent of UTIs. The present study was aimed to examine the susceptibility of UPEC isolates obtained from nosocomial cases to antibiotics, as well as their biofilm formation capability and frequency of virulence genes.

Methods

A total of 100 UPEC isolates were collected from nosocomial UTIs at Imam Reza Hospitals in Tabriz, Iran, spanning from April 2022 to January 2023. The antimicrobial susceptibility patterns were evaluated using the disk diffusion method, along with the detection of broad-spectrum β-lactam enzymes (ESBLs) and carbapenemases. The ability of isolates to form biofilms was assessed using the microtiter-plate method, while the PCR method was employed to identify the presence of virulence genes.

Results

The highest resistance was observed toward piperacillin (82%), followed by aztreonam and ciprofloxacin (81%), while the lowest resistance was found against piperacillin/tazobactam (12%) and meropenem (9%). ESBLs were detected in 62% of the isolates. The microtiter-plate results revealed strong, moderate, and weak biofilm formation abilities in 32%, 33%, and 24% of the isolates, respectively. The most prevalent virulence gene was fimA (74%) followed by hlyF (68%), papA (44%), papC (32%), iroN (26%), and cnf (20%).

Conclusion

The elevated levels of resistance to multiple antimicrobial agents, coupled with the co-presence of virulence genes and biofilm formation abilities, contribute to the persistence of UPEC-related infections, particularly in hospitalized patients. These findings underscore the necessity of implementing an effective program to control nosocomial UTIs caused by UPEC in the healthcare centers.

Abstract Image

查看原文本刊更多论文

2022-2023 年期间伊朗西北部从医院内尿路感染中分离的大肠埃希菌的毒力基因和抗生素敏感性模式：一项横断面研究。

背景和目的：尿路感染（UTI）在住院病人中很普遍，是最常见的医疗感染。尿路致病性大肠杆菌（UPEC）是尿路感染的主要致病菌。本研究旨在检测从医院病例中分离出的 UPEC 对抗生素的敏感性，以及它们形成生物膜的能力和毒力基因的频率：从2022年4月至2023年1月期间，从伊朗大不里士市伊玛目礼萨医院的医院性UTI病例中收集了100株UPEC分离株。采用磁盘扩散法评估了抗菌药敏感性模式，并检测了广谱β-内酰胺酶（ESBLs）和碳青霉烯酶。采用微孔板法评估了分离物形成生物膜的能力，同时采用 PCR 法确定了毒力基因的存在：哌拉西林的耐药性最高（82%），其次是阿曲南和环丙沙星（81%），而哌拉西林/他唑巴坦（12%）和美罗培南（9%）的耐药性最低。在 62% 的分离株中检测到了 ESBLs。微孔板结果显示，分别有 32%、33% 和 24% 的分离株具有较强、中等和较弱的生物膜形成能力。最常见的毒力基因是 fimA（74%），其次是 hlyF（68%）、papA（44%）、papC（32%）、iroN（26%）和 cnf（20%）：结论：对多种抗菌药物的耐药性水平升高，加上毒力基因和生物膜形成能力的共同存在，导致与 UPEC 相关的感染持续存在，尤其是在住院患者中。这些发现强调了在医疗中心实施有效计划控制由UPEC引起的院内UTI的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Health Science Reports Medicine-Medicine (all)

CiteScore

1.80

自引率

0.00%

发文量

458

审稿时长

20 weeks