Treatment of human sperm with GYY4137 increases sperm motility and resistance to oxidative stress.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-30 DOI:10.1017/S0967199424000340

Yan Huang, Runxin Gan, Min Zhang, Dewei Lin, Yi Cheng, Xinyu Guo

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引用次数: 0

Abstract

Hydrogen sulfide (H₂S) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing H₂S compound, on sperm damage induced by H₂O₂. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by H₂O₂. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 μM H₂O₂, followed by incubation with varying concentrations of GYY4137 (0.625-2.5 μM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that H₂O₂ adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against H₂O₂-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.

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用 GYY4137 处理人类精子可提高精子活力和抗氧化能力。

硫化氢（H2S）已被证明在各种组织和细胞的氧化应激中发挥着重要作用；然而，人们对其在精子功能中的作用仍知之甚少。本研究旨在探讨缓释硫化氢化合物 GYY4137 对 H2O2 诱导的精子损伤的保护作用。我们评估了 GYY4137 对人类精子在体外受到 H2O2 氧化损伤后的活力、存活率、脂质过氧化和 Caspase-3 活性的影响。采用密度梯度离心法选取 25 名健康男性的精子，在有或没有 10 μM H2O2 的情况下进行培养，然后用不同浓度的 GYY4137（0.625-2.5 μM）进行培养。培养 24 小时后，对精子活力、存活率、脂质过氧化和 Caspase-3 活性进行评估。结果表明，H2O2 会对精子参数产生不利影响，降低精子活力和存活率，同时增加氧化应激，表现为脂质过氧化和 caspase-3 活性升高。GYY4137 对 H2O2 诱导的氧化应激（OS）具有剂量依赖性保护作用。我们的结论是，在辅助生殖技术的体外精子制备过程中，补充 GYY4137 可提供抗氧化保护。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.