[Effect and Influencing Factors of Peripheral Blood Hematopoietic Stem Cells Collection from Unrelated Donors].

Abstract

Objective: To analyze the effect of peripheral blood hematopoietic stem cells (PBSC) collection from unrelated donors and its influencing factors.

Methods: A retrospective analysis was conducted on the mobilization and collection of PBSC from 113 unrelated donors at Yueyang Central Hospital from January 2021 to December 2023.

Results: 113 donors were successfully mobilized. The average count of PBSC mononuclear cells (MNC) and CD34⁺ cells were (12.40±7.41)×10⁸/kg and (10.64±8.07)×10⁶/kg, respectively. Univariate analysis showed that the PBSC CD34⁺ cells ratio of male donors was significantly higher than that in female donors (P =0.015). The peripheral blood (PB) white blood cell (WBC) count before collection was positively correlated with the PBSC nucleated cells count (r =0.388), and the donor's body weight, the PB CD34⁺ cell ratio before collection were positively correlated with the PBSC CD34⁺ cell ratio (r =0.259, r =0.780). The daily dose of rhG-CSF was negatively correlated with the PBSC CD34⁺ cell ratio (r =-0.285). Both rhG-CSF agents achieved successful mobilization. Multivariate analysis showed that PB WBC count before collection was a factor affecting the count of PBSC nucleated cells (P <0.001), while the PB CD34⁺cell ratio before collection was a factor affecting the PBSC CD34⁺ cell ratio (P <0.001).

Conclusion: The mobilization and collection of PBSC from unrelated donors are good, and the PB WBC count and CD34⁺ cell ratio before collection are reliable indicators for predicting the collection effect.