The function of p97/valosin-containing protein (VCP) and small VCP-interacting protein (SVIP) in invasion and migration of pancreatic cancer cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-04 eCollection Date: 2024-01-01 DOI:10.55730/1300-0144.5894

Ebru Alimoğullari, Sevil Çayli

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引用次数: 0

Abstract

Background/aim: Misfolded proteins are eliminated by a process known as endoplasmic reticulum-associated protein degradation (ERAD). ERAD has an impact on a variety of illnesses, such as diabetes, cystic fibrosis, cancer, and neurological conditions. As one of the many proteins involved in ERAD, this study is focused on p97/valosin-containing protein (VCP) and small VCP-interacting protein (SVIP). The existence and function of SVIP and p97/VCP in various types of pancreatic cancer have not yet been investigated. The study's objectives are to examine the expressions of SVIP and p97/VCP in two pancreatic cancer types and to show whether these proteins aid in the invasion and migration of pancreatic cancer cells.

Materials and methods: In this work, MIA PaCa-2 and PANC-1 human cell lines were examined. Immunocytochemistry and immunofluorescence were performed to detect the cellular localization and presence of p97/VCP and SVIP in pancreatic cancer cells. Following p97/VCP siRNA and SVIP siRNA transfection of the cells, protein expressions were assessed using Western blot analysis. The effects of this suppression on cell invasion and migration were determined using the xCELLigence real-time analysis system (RTAC).

Results: In the nucleus and cytoplasm of MIA PaCa-2 and PANC-1 cells, p97/VCP and SVIP immunoexpressions were seen. The decrease in protein expressions of p97/VCPsi and SVIPsi was significant in pancreatic cells compared to the controlsi. The p97/VCP siRNA transfection reduced the invasion and migration of MIA PaCa-2 and PANC-1 cells. In addition, the SVIP siRNA suppression resulted in increasing the invasion and migration ability of both cells. This study also demonstrated, for the first time, SVIP expression in MIA PaCa-2 and PANC-1 cells.

Conclusion: Overall, the findings show the differential expression and function of p97/VCP and SVIP in pancreas ductal adenocarcinoma cells. The potential of the pancreatic cancer cells to migrate and invade altered when the two cell lines were transfected with p97/VCPsi and SVIPsi.

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p97/valosin-containing protein (VCP) 和小 VCP-interacting protein (SVIP) 在胰腺癌细胞侵袭和迁移中的功能。

背景/目的：错误折叠的蛋白质通过一个称为内质网相关蛋白质降解（ERAD）的过程被消除。ERAD对糖尿病、囊性纤维化、癌症和神经系统疾病等多种疾病都有影响。作为参与ERAD的众多蛋白之一，本研究主要关注p97/含缬氨酸蛋白（VCP）和小VCP相互作用蛋白（SVIP）。目前尚未研究 SVIP 和 p97/VCP 在各种类型胰腺癌中的存在和功能。本研究的目的是检测 SVIP 和 p97/VCP 在两种胰腺癌类型中的表达，并说明这些蛋白是否有助于胰腺癌细胞的侵袭和迁移：在这项工作中，对 MIA PaCa-2 和 PANC-1 人细胞系进行了研究。免疫细胞化学和免疫荧光检测了 p97/VCP 和 SVIP 在胰腺癌细胞中的定位和存在。p97/VCP siRNA 和 SVIP siRNA 转染细胞后，使用 Western 印迹分析评估蛋白质表达。使用 xCELLigence 实时分析系统（RTAC）测定了这种抑制对细胞侵袭和迁移的影响：结果：在MIA PaCa-2和PANC-1细胞的细胞核和细胞质中，均可见p97/VCP和SVIP免疫表达。与对照组相比，p97/VCPsi 和 SVIPsi 在胰腺细胞中的蛋白表达明显减少。p97/VCP siRNA 转染降低了 MIA PaCa-2 和 PANC-1 细胞的侵袭和迁移。此外，SVIP siRNA的抑制作用也增强了这两种细胞的侵袭和迁移能力。本研究还首次证实了 SVIP 在 MIA PaCa-2 和 PANC-1 细胞中的表达：总之，研究结果显示了 p97/VCP 和 SVIP 在胰腺导管腺癌细胞中的不同表达和功能。当两个细胞系转染 p97/VCPsi 和 SVIPsi 时，胰腺癌细胞迁移和侵袭的潜力发生了改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.