Characterisation of a new virulent phage isolated from Hainan Island with potential against multidrug-resistant Pseudomonas aeruginosa infections

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2025-01-01 DOI:10.1016/j.resmic.2024.104250

Anyang Li , Chen Chen , Yanmei Li , Yanshuang Wang , Xuemiao Li , Qiao Zhu , Yue Zhang , Shen Tian , Qianfeng Xia

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Abstract

Multidrug-resistant (MDR) Pseudomonas aeruginosa is a serious life-threatening pathogen. The rise in P. aeruginosa resistance rates has renewed interest in phages as an alternative therapeutic approach for treating bacterial infections. In this study, we investigated the characteristics of the first Pseudomonas phage, vB_PaP_HN01, isolated from Hainan, the only tropical island in China. The lytic rate of this phage against P. aeruginosa reached 64.3 % (27/42). Under the optimal multiplicity of infection (MOI) of 0.1, more than 90 % of phage particles absorb onto the host cell within 10 min, with an eclipse period of around 15 min, and a high titer phage production (10¹¹ PFU/ml) within 90 min was demonstrated. vB_PaP_HN01 maintains a robust titer after 1 h exposure to pH values and temperatures (up to 50 °C). Genome annotation revealed that vB_PaP_HN01 did not contain drug-resistance or lysogeny-associated genes. It can effectively inhibit the formation of biofilms of MDR P. aeruginosa and eliminated aggressive biofilms (removal rate about 70 %). In the in vivo infection models, it was demonstrated that the survival rate and lifespan of Galleria mellonella larvae were increased alongside the injection of vB_PaP_HN01. These data revealed the potential of vB_PaP_HN01 against P. aeruginosa in clinic.

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从海南岛分离出的一种新型毒性噬菌体的特征，该噬菌体具有抗耐多药型铜绿假单胞菌感染的潜力。

耐多药（MDR）铜绿假单胞菌是一种严重威胁生命的病原体。铜绿假单胞菌耐药率的上升再次激发了人们对噬菌体作为治疗细菌感染的替代疗法的兴趣。在这项研究中，我们研究了从中国唯一的热带岛屿海南分离出来的第一个假单胞菌噬菌体 vB_PaP_HN01 的特性。该噬菌体对铜绿假单胞菌的致死率达到 64.3%（27/42）。在最佳感染倍数（MOI）为 0.1 的情况下，超过 90% 的噬菌体颗粒在 10 分钟内吸收到宿主细胞上，食期约为 15 分钟，并在 90 分钟内产生高滴度噬菌体（1011 PFU/ml）。vB_PaP_HN01 在 pH 值（3-11）和温度（高达 50°C）下暴露 1 小时后仍能保持稳定的滴度。基因组注释显示，vB_PaP_HN01 不含抗药性基因或溶菌相关基因。它能有效抑制 MDR 铜绿假单胞菌生物膜的形成，并消除侵袭性生物膜（去除率约为 70%）。在体内感染模型中，结果表明在注射 vB_PaP_HN01 的同时，铜绿微囊藻幼虫的存活率和寿命也得到了提高。这些数据揭示了 vB_PaP_HN01 在临床上抗击铜绿假单胞菌的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.