Comparative transcriptomic analysis unveils candidate genes associated with sugarcane growth rate.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-29 DOI:10.1007/s00425-024-04555-3
Yegeng Fan, Huiwen Zhou, Haifeng Yan, Aomei Li, Lihang Qiu, Zhongfeng Zhou, Yuchi Deng, Rongfa Chen, Jianming Wu
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Abstract

Sugarcane (Saccharum spp.) growth is regulated by intricate gene networks and hormone secretions, positively correlating with sugarcane yield. There is a rising interest in exploring how the candidate genes found in sugarcane respond to plant growth. In this study, we simulated a typical growth environment to obtain accurate phenotypic data and screened for potential genes associated with plant growth through transcriptomics. Compared to Saccharum GuiTang 42, the other variety Saccharum GuiTang 44 exhibited earlier germination, a higher emergence rate, thicker pseudostems, taller plants, and a more extensive root system. The middle buds formed the greatest number of roots, followed by the lower and upper buds. Indole-3-acetic acid (IAA) and jasmonic acid effectively promoted bud development, while abscisic acid and trans-zeatin exhibited negative correlations with sugarcane bud growth. Transcriptome data from the upper, middle, and lower buds revealed 24,158 differentially expressed genes in all three comparisons, with MAPK signaling emerging as a critical pathway. The photosynthesis-antenna protein pathway is vital for middle and lower bud development during root germination. Lastly, key gene modules related to differences in hormone content between the two varieties were defined through weighted correlation network analysis and identified. The module significantly associated with IAA was enriched in pathways such as Proteasome and Protein processing in the endoplasmic reticulum, and the upregulation of key genes involved in this gene module had a highly significant positive correlation with bud outgrowth combined with IAA secretion. In conclusion, we have elucidated the pathways of hormones during sugarcane growth and the interactions between IAA and critical genes. These in-depth findings may guide modern sugarcane breeding.

比较转录组分析揭示了与甘蔗生长速度相关的候选基因。
甘蔗(Saccharum spp.)的生长受复杂的基因网络和激素分泌的调控,与甘蔗产量呈正相关。探索甘蔗中发现的候选基因如何对植物生长做出反应的兴趣日益高涨。在本研究中,我们模拟了典型的生长环境,以获得准确的表型数据,并通过转录组学筛选与植物生长相关的潜在基因。与 "桂糖42号 "相比,另一个品种 "桂糖44号 "发芽更早、出苗率更高、假茎更粗、植株更高、根系更广。中芽形成的根数量最多,其次是下芽和上芽。吲哚-3-乙酸(IAA)和茉莉酸能有效促进芽的发育,而脱落酸和反玉米素则与甘蔗芽的生长呈负相关。上芽、中芽和下芽的转录组数据显示,在所有三种比较中,有 24 158 个基因表达不同,其中 MAPK 信号转导是一个关键途径。光合作用-天线蛋白途径对根萌发期间中下部芽的发育至关重要。最后,通过加权相关网络分析,确定了与两个品种激素含量差异相关的关键基因模块。与IAA明显相关的模块富集在蛋白酶体和内质网蛋白质加工等通路中,该基因模块所涉及的关键基因的上调与芽的萌发和IAA的分泌有非常明显的正相关。总之,我们阐明了甘蔗生长过程中激素的作用途径以及 IAA 与关键基因之间的相互作用。这些深入研究结果可为现代甘蔗育种提供指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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