Survey of sand fly fauna in six provinces of Southern Vietnam with species identification using DNA barcoding.

IF 3 2区 医学 Q1 PARASITOLOGY
Trang Thuy Nguyen, Yudhi Ratna Nugraheni, Hoang Lan Anh Nguyen, Apinya Arnuphapprasert, Theerakamol Pengsakul, Le Quang Thong, Rinnara Ampol, Padet Siriyasatien, Morakot Kaewthamasorn
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引用次数: 0

Abstract

Background: Sand flies, belonging to the Psychodidae family, represent small, hairy insects that serve as significant vectors in various important medical and veterinary diseases. Despite being recognized by the World Health Organization as an endemic area for leishmaniasis, Southeast Asia lacks comprehensive information on the species composition and biology of sand flies. To address this, the current study aimed to survey sand fly biodiversity.

Methods: Sand flies from six provinces in Southern Vietnam were collected using CDC light traps. Sand flies were subsequently identified morphologically and confirmed molecularly using mitochondrial cytochrome oxidase c subunit I (COI) and cytochrome b (cytb) sequences. BLASTN searches were conducted, and the species identity of sand flies was further confirmed through a Barcode of Life Database (BOLD) search utilizing COI sequences. Subsequently, nucleotide sequences were subjected to a panel of analyses including intraspecific variation, phylogenetic relationships and haplotype network. The average densities of collected sand flies (sand flies/trap/night) and species richness were also recorded.

Results: A total of 753 sand flies were collected. After excluding damaged specimens, six sand fly species, namely Phlebotomus stantoni, Sergentomyia khawi, Se. silvatica, Se. barraudi, Se. bailyi and Grassomyia indica, were identified. All conspecific sand fly sequences, including Ph. stantoni, Se. barraudi, Gr. indica, Se. bailyi, Se. khawi and Se. silvatica, clustered with their reference sequences, corroborating the results of morphology-based identification, BLASTN analysis and BOLD search. For intraspecific variation of sand flies obtained from the current study, COI diversity indices were consistently higher than those of cytb.

Conclusions: This study provides the first updates on morphological and molecular characterization of sand flies in Southern Vietnam. This acquired knowledge on sand fly species composition is essential for controlling sand fly-borne diseases in this potentially endemic region.

越南南部六省沙蝇动物群调查及 DNA 条形码物种鉴定。
背景:沙蝇属于灵蝇科,是一种小型多毛昆虫,是各种重要的医疗和兽医疾病的重要传播媒介。尽管世界卫生组织认定东南亚是利什曼病的流行区,但却缺乏有关沙蝇物种组成和生物学特性的全面信息。为解决这一问题,本研究旨在调查沙蝇的生物多样性:方法:使用 CDC 灯光诱捕器收集越南南部六个省的沙蝇。随后对沙蝇进行了形态鉴定,并利用线粒体细胞色素氧化酶 c 亚单位 I(COI)和细胞色素 b(cytb)序列对其进行了分子鉴定。进行了 BLASTN 搜索,并通过利用 COI 序列的生命条形码数据库(BOLD)搜索进一步确认了沙蝇的物种身份。随后,对核苷酸序列进行了一系列分析,包括种内变异、系统发育关系和单倍型网络。此外,还记录了收集到的沙蝇的平均密度(沙蝇/诱捕器/夜)和物种丰富度:结果:共收集到 753 只沙蝇。在剔除损坏的标本后,共鉴定出 6 个沙蝇物种,即 Phlebotomus stantoni、Sergentomyia khawi、Se. silvatica、Se. barraudi、Se. bailyi 和 Grassomyia indica。所有同种沙蝇序列,包括 Ph. stantoni、Se. barraudi、Gr. indica、Se. bailyi、Se. khawi 和 Se. silvatica,均与其参考序列聚类,证实了基于形态学鉴定、BLASTN 分析和 BOLD 搜索的结果。在本研究获得的沙蝇种内变异中,COI 多样性指数一直高于 cytb 指数:本研究首次更新了越南南部沙蝇的形态和分子特征。这些关于沙蝇物种组成的知识对于在这一潜在的地方病流行地区控制沙蝇传播的疾病至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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