The dysregulation and clinical relevance of lncRNAs MYOSLID and SFTA1P in colorectal cancer patients.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2024-10-30 DOI:10.1007/s11033-024-10020-x

Amir Reza Karamzadeh, Mansour Heidari, Abolfazl Namazi, Seidamir Pasha Tabaeian, Abolfazl Akbari

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引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is a very common cancer worldwide. CRC is characterized by some changes in the expression of oncogenic and tumor suppressor genes. These changes are associated with dysregulation of non-coding RNAs, including long non-coding RNAs (lncRNAs). LncRNAs are heterogeneous non-coding molecules without open reading frames. LncRNAs have been established as regulators in the development of CRC and clinical biomarkers for the CRC detection. In this project, we investigated the expression changes of two new lncRNAs named SFTA1P and MYOSLID in CRC patients.

Materials and methods: 30 samples of CRC tissue and 30 samples of normal tissue adjacent to the cancer tissue were obtained from patients. RNA extraction from tissue samples was performed using RNAX plus. ExcelRT™ Reverse Transcription Kit (SymBio, Korea) was used for cDNA synthesis. RealQ Plus 2x Master Mix Green Without ROX™ was used to perform a quantitative PCR (qPCR). REST, and SPSS software were used for statistical analysis.

Result: Our result demonstrated that lncRNAs MYOSLID and SFTA1P were significantly up-regulated in tumor tissues compared to healthy tissues with a fold change of 13.43 and 5.33 (P < 0.05) respectively. Based on the analysis of ROC curve, MYOSLID (AUC = 0.946, P < 0.0001, SE =0.0035) and SFTA1P (AUC = 0.800, P < 0.0001, SE = 0.059) were indicated as potential clinical hallmarks for CRC patients.

Conclusion: According to the results obtained from this research, lncRNAs SFTA1P and MYOSLID can be suggested as molecular biomarkers for the CRC diagnosis.

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lncRNAs MYOSLID和SFTA1P在结直肠癌患者中的失调及其临床意义。

背景：结直肠癌（CRC）是世界上一种非常常见的癌症。CRC 的特点是致癌基因和抑癌基因的表达发生了一些变化。这些变化与非编码 RNA（包括长非编码 RNA，lncRNA）的失调有关。LncRNA 是没有开放阅读框的异质性非编码分子。LncRNAs 已被确定为 CRC 发展过程中的调控因子和 CRC 检测的临床生物标志物。在本项目中，我们研究了名为 SFTA1P 和 MYOSLID 的两个新 lncRNA 在 CRC 患者中的表达变化。组织样本的 RNA 提取采用 RNAX plus.使用 ExcelRT™ 逆转录试剂盒（韩国 SymBio 公司）进行 cDNA 合成。RealQ Plus 2x Master Mix Green Without ROX™ 用于进行定量 PCR（qPCR）。使用 REST 和 SPSS 软件进行统计分析：结果表明，与健康组织相比，lncRNAs MYOSLID 和 SFTA1P 在肿瘤组织中明显上调，其折叠变化分别为 13.43 和 5.33（P根据研究结果，lncRNAs SFTA1P和MYOSLID可作为诊断CRC的分子生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.