Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-10-30 DOI:10.1002/jbm.b.35493

M. L. Torres, A. Hurtado Cuba, T. G. Oberti, J. M. Fernandez

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Abstract

In the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.

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藻酸盐-HEMA 水凝胶作为有望用于骨再生的生物材料：体外和体内研究。

在本研究中，研究了之前开发的半互穿 HEMA-EGDMA 聚合物网络（sIPN）在骨组织工程（BTE）中应用的成骨和血管生成特性。使用大鼠骨髓祖细胞（BMPCs）、EA.hy926 内皮细胞和大鼠血管平滑肌细胞（VSMCs）进行了体外表征研究。根据这项工作和以前工作的体外研究结果，我们选择了水凝胶进行体内研究，利用骨再生模型了解水凝胶作为生物材料的能力。我们的研究结果表明，在 HEMA-EGDMA 聚合物网络中加入海藻酸盐可分别促进 BMPCs 和 EA.hy926 以及 VSMCs 细胞培养物的成骨和血管生成能力，在体内研究中还可增加骨形成和血管结构，这证明了其作为生物材料在 BTE 中的潜在用途。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.