Anti-inflammatory effects of a methanol extract from Montanoa grandiflora DC. (Asteraceae) leaves on in vitro and in vivo models.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2024-10-29 DOI:10.1007/s10787-024-01573-1

Mariana Sánchez-Canul, Fabiola Villa-de la Torre, Rocío Borges-Argáez, Claribel Huchin-Chan, Guillermo Valencia-Pacheco, Eunice Yáñez-Barrientos, Michelle Romero-Hernández, Angel Josabad Alonso-Castro, Víctor Ermilo Arana-Argáez

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引用次数: 0

Abstract

Background: Montanoa grandiflora, a plant species native from Mexico to Central America, locally known as "Teresita" in Yucatán, México, is used to alleviate anxiety, rheumatism, and stomach issues. This study aims to investigate the anti-inflammatory properties of the methanol extract of Montanoa grandiflora leaves (MMG) in experimental models of inflammation.

Methods: Gas chromatography-mass spectroscopy was used to characterize the MMG; cytotoxicity was assessed by MTT assay on murine macrophages and hemolysis assay. The in vitro anti-inflammatory activity was evaluated on LPS-stimulated murine macrophages by measuring of pro- and anti-inflammatory cytokines, NO and H₂O₂ release. The in vivo anti-inflammatory activity was evaluated using carrageenan-induced mouse paw edema, 12-O-tetradecanoylphorbol 13-acetate induced-ear edema, and 1-fluoro-2,4-dinitrobenzene induced-delayed-type hypersensitivity. In addition, the serum levels of prostaglandins and leukotrienes were assessed.

Results: The main compounds found in MMG were terpenes (i.e., β-caryophyllene, (-)-α-cubebene, alloaromadendrene, ( +)-δ-cadinene, β-eudesmol), alkaloid (( ±)-nor-β-hydrastine), cyclic polyol (quinic acid), carbohydrates and their derivatives, and fatty acids (octadecatrienoic acid and octadecanoic acid). MMG did not exhibit cytotoxic or hemolytic activity. However, it demonstrated in vitro anti-inflammatory effects by increasing the production of IL-10, decreasing the levels of TNF-α, IL-1β, IL-6, NO and H₂O₂. MMG significantly reduced carrageenan-induced paw edema, TPA-induced ear edema, and DNFB-induced delayed-type hypersensitivity in mice with effects comparable to those of standard drugs, as well as serum levels of prostaglandins and leukotrienes.

Conclusion: The anti-inflammatory activity of MMG is associated with increased IL-10 levels and inhibiting inflammatory cell migration mechanisms, without causing cytotoxic or hemolytic damage in both in vitro and in vivo assays.

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Montanoa grandiflora DC.（菊科）叶片甲醇提取物对体外和体内模型的抗炎作用。

背景介绍Montanoa grandiflora是一种原产于墨西哥和中美洲的植物物种，在墨西哥尤卡坦州当地被称为 "Teresita"，可用于缓解焦虑、风湿和胃病。本研究旨在调查在炎症实验模型中蒙塔诺叶甲醇提取物（MMG）的抗炎特性：方法：采用气相色谱-质谱法对 MMG 进行表征；通过 MTT 试验和溶血试验对小鼠巨噬细胞进行细胞毒性评估。通过测量促炎和抗炎细胞因子、NO 和 H2O2 的释放，评估了 LPS 刺激的小鼠巨噬细胞的体外抗炎活性。使用卡拉胶诱导的小鼠爪水肿、12-O-十四碳酰樟脑酚-13-乙酸酯诱导的耳水肿和 1-氟-2,4-二硝基苯诱导的延迟型超敏反应评估了其体内抗炎活性。此外，还评估了血清中前列腺素和白三烯的水平：结果：在 MMG 中发现的主要化合物是萜烯类化合物（即 β-茶叶烯、(-)-α-椰油烯、alloaromadendrene、(+)-δ-cadinene、β-桉叶油醇）、生物碱（( ±)-nor-β-hydrastine ）、环多元醇（奎宁酸）、碳水化合物及其衍生物和脂肪酸（十八碳三烯酸和十八烷酸）。MMG 不具有细胞毒性或溶血活性。不过，它具有体外抗炎作用，能增加 IL-10 的产生，降低 TNF-α、IL-1β、IL-6、NO 和 H2O2 的水平。MMG能明显减轻卡拉胶诱导的小鼠爪水肿、TPA诱导的小鼠耳水肿和DNFB诱导的小鼠迟发型超敏反应，其效果与标准药物相当，同时还能降低血清中前列腺素和白三烯的水平：结论：MMG的抗炎活性与IL-10水平升高和抑制炎症细胞迁移机制有关，在体外和体内试验中不会造成细胞毒性或溶血性损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]