Improvement of RBD-FC Immunogenicity by Using Alum–Sodium Alginate Adjuvant Against SARS-COV-2

IF 4.3 4区医学 Q1 INFECTIOUS DISEASES

Influenza and Other Respiratory Viruses Pub Date : 2024-10-30 DOI:10.1111/irv.70018

Mahboobeh Dehghan, Hossein Askari, Masoud Tohidfar, Seyed Omid Ranaei Siadat, Fataneh Fatemi

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Abstract

Background

Adjuvants use several mechanisms to boost immunogenicity and to modulate immune response. The strength of adsorption of antigen by adjuvants can be a determinant factor for significant improvement of immunopotentiation.

Methods

We expressed recombinant RBD-FC in PichiaPink Strain 4 and examined the vaccination of mice by vaccine formulation with different adjuvants (sodium alginate and aluminum hydroxide, alone and together).

Results

Sodium alginate significantly increased the immunogenicity and stability of RBD-FC antigen, so RBD-FC formulated with combined alginate and alum (AlSa) and sodium alginate alone showed higher antibody titer and stability. Immunogenicity of RBD-FC:AlSa was determined by serological assays including direct enzyme-linked immunosorbent assay (ELISA) and surrogate virus neutralization test (sVNT). High levels of IgGs and neutralizing antibodies were measured in serum of mice immunized with the RBD-FC:AlSa formulation. On the other hand, cytokines IL-10 and INF-γ were severely accumulated in response to RBD-FC:AlSa, and after 10 days, their accumulation was significantly declined, whereas IL-4 showed the highest and the lowest accumulation in response to alum and alginate, respectively.

Conclusions

Our data may suggest that combination of alum and sodium alginate has a better compatibility with RBD-FC in vaccine formulation.

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使用海藻酸钠明矾佐剂提高 RBD-FC 对 SARS-COV-2 的免疫原性

背景：佐剂利用多种机制增强免疫原性和调节免疫反应。佐剂对抗原的吸附强度是显著提高免疫增强作用的决定性因素：方法：我们在 PichiaPink Strain 4 中表达了重组 RBD-FC，并研究了不同佐剂（海藻酸钠和氢氧化铝，单独使用或共同使用）的疫苗配方对小鼠的接种效果：结果：海藻酸钠明显增加了 RBD-FC 抗原的免疫原性和稳定性，因此海藻酸钠和明矾（AlSa）混合配制的 RBD-FC 和单独使用海藻酸钠配制的 RBD-FC 显示出更高的抗体滴度和稳定性。RBD-FC:AlSa 的免疫原性是通过血清学检测确定的，包括直接酶联免疫吸附试验（ELISA）和替代病毒中和试验（sVNT）。用 RBD-FC:AlSa 制剂免疫的小鼠血清中测得了高水平的 IgG 和中和抗体。另一方面，细胞因子 IL-10 和 INF-γ 在 RBD-FC:AlSa 的反应中严重积累，10 天后积累量明显下降，而 IL-4 在明矾和海藻酸的反应中分别显示出最高和最低的积累量：我们的数据可能表明，明矾和海藻酸钠的组合与 RBD-FC 在疫苗制剂中具有更好的相容性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Influenza and Other Respiratory Viruses 医学-病毒学

CiteScore

7.20

自引率

4.50%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Influenza and Other Respiratory Viruses is the official journal of the International Society of Influenza and Other Respiratory Virus Diseases - an independent scientific professional society - dedicated to promoting the prevention, detection, treatment, and control of influenza and other respiratory virus diseases. Influenza and Other Respiratory Viruses is an Open Access journal. Copyright on any research article published by Influenza and Other Respiratory Viruses is retained by the author(s). Authors grant Wiley a license to publish the article and identify itself as the original publisher. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.