Classification of epidermal, buccal, penile and vaginal epithelial cells using morphological characteristics measured by imaging flow cytometry

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
Dana Ross , Duncan Taylor , Roland A.H. van Oorschot , Giles Best , Mariya Goray
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Abstract

As a result of the increased sensitivity of forensic DNA techniques, which can generate informative results from as little as a few cells, developing an understanding of the anatomical region these cells originate from is becoming more pertinent. Imaging Flow Cytometry (IFC) represents a promising method for identifying epithelial cells from different anatomical regions. This project aimed to determine whether IFC could be used to distinguish epithelial cells collected from different forensically relevant anatomical regions based on their morphology and autofluorescence. Penile, vaginal, buccal, and epidermal epithelial cells were collected in triplicate from 15 male and 15 female participants, in three different age groups: 18–39, 40–59, and 60+ years. Using the high statistical output from the IFC, 234 morphological measurements were collected for 571,546 single cells. Using a linear discriminate analysis with a minimum posterior probability threshold, the four epithelial cell types could be identified and distinguished with a 72–83 % classification accuracy. The results showed that the age and biological sex of the individual had no effect on the morphology of the four epithelial cell types. These data provide insights into the ability of IFC to identify and distinguish penile, buccal, vaginal, and epidermal epithelial cells and identifies further avenues for improvement and optimisation.
利用成像流式细胞仪测量的形态特征对表皮、口腔、阴茎和阴道上皮细胞进行分类。
由于法医 DNA 技术的灵敏度不断提高,只需几个细胞就能得出翔实的结果,因此了解这些细胞来自哪个解剖区域变得越来越重要。成像流式细胞术(IFC)是识别不同解剖区域上皮细胞的一种有前途的方法。本项目旨在确定 IFC 是否可用于根据形态和自发荧光来区分从不同法医相关解剖区域收集的上皮细胞。从 15 名男性和 15 名女性参与者身上收集了阴茎、阴道、口腔和表皮上皮细胞,这些细胞一式三份,分别来自三个不同的年龄组:18-39 岁、40-59 岁和 60 岁以上。利用 IFC 的高统计输出,对 571,546 个单细胞进行了 234 项形态测量。利用最小后验概率阈值的线性判别分析,可以识别和区分四种上皮细胞类型,分类准确率为 72-83%。结果表明,个体的年龄和生理性别对四种上皮细胞类型的形态没有影响。这些数据有助于深入了解 IFC 识别和区分阴茎、口腔、阴道和表皮上皮细胞的能力,并确定了进一步改进和优化的途径。
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来源期刊
Forensic science international
Forensic science international 医学-医学:法
CiteScore
5.00
自引率
9.10%
发文量
285
审稿时长
49 days
期刊介绍: Forensic Science International is the flagship journal in the prestigious Forensic Science International family, publishing the most innovative, cutting-edge, and influential contributions across the forensic sciences. Fields include: forensic pathology and histochemistry, chemistry, biochemistry and toxicology, biology, serology, odontology, psychiatry, anthropology, digital forensics, the physical sciences, firearms, and document examination, as well as investigations of value to public health in its broadest sense, and the important marginal area where science and medicine interact with the law. The journal publishes: Case Reports Commentaries Letters to the Editor Original Research Papers (Regular Papers) Rapid Communications Review Articles Technical Notes.
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