Global cell-free DNA methylation in patients with active tuberculosis and tuberculosis contacts with latent tuberculosis infection

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-18 DOI:10.1016/j.diagmicrobio.2024.116559

Chih-Jung Chang , Jhong-Ru Huang , Yen-Han Tseng , Sheng-Wei Pan , Jia-Yih Feng , Wei-Juin Su , Yuh-Min Chen

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引用次数: 0

Abstract

Introduction

To investigate whether the methylation of circulating cell-free DNA (cfDNA) differentiates active tuberculosis (TB) from latent TB infection (LTBI).

Methods

Patients with pulmonary TB, contacts with LTBI, and healthy controls were enrolled (2018–2021). Plasma cfDNA was extracted, and using a 5-methylcytosine (5mC) DNA ELISA kit, the global methylation of cfDNA (5mC-cfDNA) was measured.

Results

59 TB, 63 LTBI, 39 healthy controls were included. The 5mC-cfDNA level was higher in TB (6.4 %) than LTBI (4.1 %) and healthy controls (4.9 %) (both p<0.05). Independent TB factors were 5mC-cfDNA ≥6.6 % and CRP ≥0.32 mg/dL (adjusted odds ratio (aOR) 4.594 [95 % CI:1.628–12.965], p=0.004 and 5.338 [1.659–17.176], p=0.005). Having one or both factors increased TB odds 8- and 16-fold (aOR 8.688 [3.229–23.378], p <0.001 and 16.080 [3.092–83.632], p =0.001).

Conclusion

The global cfDNA methylation level was higher in TB than contacts without TB and helped differentiate patients with TB from contacts with LTBI.

查看原文本刊更多论文

活动性肺结核患者和肺结核潜伏感染者的全球无细胞 DNA 甲基化。

简介：目的：研究循环游离细胞 DNA（cfDNA）的甲基化是否能区分活动性结核病（TB）和潜伏性结核感染（LTBI）：研究循环无细胞DNA（cfDNA）的甲基化是否能区分活动性肺结核（TB）和潜伏性肺结核感染（LTBI）：入组肺结核患者、LTBI接触者和健康对照组（2018-2021年）。提取血浆cfDNA，使用5-甲基胞嘧啶（5mC）DNA ELISA试剂盒测定cfDNA的全局甲基化（5mC-cfDNA）：结果：59 名肺结核患者、63 名长期慢性阻塞性肺病患者和 39 名健康对照者被纳入研究。结核病患者的 5mC-cfDNA 水平（6.4%）高于长期慢性阻塞性肺病患者（4.1%）和健康对照组（4.9%）（均为 p）：肺结核患者的 cfDNA 整体甲基化水平高于无肺结核的接触者，有助于区分肺结核患者和长期慢性阻塞性肺病接触者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.