Effect of bacterial dissociation on lipopolysaccharide structure: A study of O-polysaccharide from the marine bacterium Pseudoalteromonas agarivorans KMM 232 (O-form)

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-11-01 DOI:10.1016/j.carres.2024.109300

Maxim S. Kokoulin , Vlada S. Belova , Lyudmila A. Romanenko

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Abstract

The lipopolysaccharide (LPS) was obtained from a bacterium Pseudoalteromonas agarivorans KMM 232 (O-form) isolated from a seawater sample collected at a depth of 500 m. The O-polysaccharide (OPS) was isolated by mild acid degradation of the LPS and studied by chemical methods along with 1D and 2D ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, ¹H,¹H TOCSY, ¹H,¹H ROESY and ¹H,¹³C HSQC, and ¹H,¹³C HMBC experiments. The following new structure of the OPS from P. agarivorans KMM 232 (O-form) containing 2-acetamido-2-deoxy-d-glucose (D-GlcNAc), d-glucose (D-Glc), d-glucuronic acid (D-GlcA), 4,6-O-[(R)-1-carboxyethylidene]-d-galactose [D-Galp4,6 (R-Pyr)] and two residues of d-galactose (D-Gal) was established:

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细菌解离对脂多糖结构的影响：对海洋细菌 Pseudoalteromonas agarivorans KMM 232（O-form）O 型多糖的研究。

脂多糖（LPS）取自从 500 米深处采集的海水样本中分离出的一种假交替单胞菌（Pseudoalteromonas agarivorans KMM 232）（O-form）。通过弱酸降解 LPS 分离出了 O 型多糖（OPS），并通过化学方法以及一维和二维 1H 和 13C NMR 光谱（包括 1H,1H COSY、1H,1H TOCSY、1H,1H ROESY 和 1H,13C HSQC 以及 1H,13C HMBC 实验）对其进行了研究。以下是来自 P. agarivorans KMM 232（O-form）的 OPS 的新结构，其中含有 2-乙酰氨基-2-脱氧-d-葡萄糖（D-GlcNAc）、d-葡萄糖（D-Glc）、d-葡萄糖醛酸（D-GlcA）、4,6-O-[(R)-1-羧基亚乙基]-d-半乳糖[D-Galp4,6 (R-Pyr)]和两个 d-半乳糖残基（D-Gal）。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".