Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-30 DOI:10.1186/s12870-024-05728-8

Li Yang, Lingli Yang, Chuanji Zhao, Zetao Bai, Meili Xie, Jie Liu, Xiaobo Cui, Klaas Bouwmeester, Shengyi Liu

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引用次数: 0

Abstract

Background: Rapeseed (Brassica napus L.) is an important oil and industrial crop worldwide. Black rot caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc) is an infectious vascular disease that leads to considerable yield losses in rapeseed. Resistance improvement through genetic breeding is an effective and sustainable approach to control black rot disease in B. napus. However, the molecular mechanisms underlying Brassica-Xcc interactions are not yet fully understood, especially regarding the impact of post-transcriptional gene regulation via alternative splicing (AS).

Results: In this study, we compared the AS landscapes of a susceptible parental line and two mutagenized B. napus lines with contrasting levels of black rot resistance. Different types of AS events were identified in these B. napus lines at three time points upon Xcc infection, among which intron retention was the most common AS type. A total of 1,932 genes was found to show differential AS patterns between different B. napus lines. Multiple defense-related differential alternative splicing (DAS) hub candidates were pinpointed through an isoform-based co-expression network analysis, including genes involved in pathogen recognition, defense signalling, transcriptional regulation, and oxidation reduction.

Conclusion: This study provides new insights into the potential effects of post-transcriptional regulation on immune responses in B. napus towards Xcc attack. These findings could be beneficial for the genetic improvement of B. napus to achieve durable black rot resistance in the future.

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揭示野油菜黄单胞菌感染下易感和抗病甘蓝品系的替代剪接模式。

背景：油菜籽（Brassica napus L.）是全球重要的油料和工业作物。由细菌病原体野油菜黄单胞菌（Xanthomonas campestris pv.campestris，Xcc）引起的黑腐病是一种传染性维管束病害，会导致油菜大量减产。通过遗传育种提高抗病性是控制油菜黑腐病的有效且可持续的方法。然而，芸苔属植物与黑腐病相互作用的分子机制尚未完全清楚，特别是通过替代剪接（AS）进行转录后基因调控的影响：结果：在这项研究中，我们比较了一个易感亲本品系和两个具有不同黑腐病抗性水平的诱变油菜品系的 AS 图谱。结果：我们比较了易感亲本品系和两个黑腐病抗性水平截然不同的诱变油菜品系的AS景观。在这些油菜品系中，Xcc感染后的三个时间点发现了不同类型的AS事件，其中内含子保留是最常见的AS类型。在不同的油菜品系中，共有 1,932 个基因表现出不同的 AS 模式。通过基于同工酶的共表达网络分析，确定了多个与防御相关的差异替代剪接（DAS）中心候选基因，包括涉及病原体识别、防御信号、转录调控和氧化还原的基因：本研究为转录后调控对油菜对 Xcc 攻击的免疫反应的潜在影响提供了新的见解。这些发现可能有利于油菜的遗传改良，从而在未来获得持久的黑腐病抗性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.