Hypoglycemic Assessment of Aqueous Leaf Extract of Moringa oleifera on Diabetic Wistar Rats.

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI:10.1155/2024/9779021
Egbujo Ejike Amina, James O Adisa, Solomon Matthias Gamde, Etinosa Beauty Omoruyi, Habauka M Kwaambwa, Lamech M Mwapagha
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引用次数: 0

Abstract

Background: Moringa oleifera leaf is used for diabetes due to its pharmacologic effects. Patients with hyperglycemia experience beta cell destruction. However, no research on risk awareness has been done to ascertain its safety. The present study describes the antidiabetic effect of Moringa oleifera leaf, such as the protection of pancreatic beta cells and the induction of glycogen synthesis, before addressing the secondary effects of diabetes, such as hepatic and renal toxicity. Methods: Forty-five Wistar rats weighed 160 ± 10 g were divided into nine groups. All animal operations complied with the National Institute of Health (NIH) guidelines for the care and use of laboratory animals as approved by the Animal Ethical Committee, University of Jos. Group I was normal control and Group II was diabetic animals induced with alloxan. Insulin and extract doses of 200, 400, and 800 mg/kg were given to diabetic Groups III-VI. Normal animals in Groups VII-IX were given extract at doses of 200, 400, and 800 mg/kg for 28 days. Tissues were retrieved for biochemical and histological investigations using standard techniques. Results: There was decrease relative body weight of diabetic animals (95.50 ± 5.50) when compared to normal control (142.75 ± 20.08) with increased levels of urea (control 6.13 ± 0.523 and diabetes 29.23 ± 1.267) and creatinine (control 0.70 ± 0.057 and diabetes 2.13 ± 0.185). Histology of the liver and pancreas also points to organ damage due to hyperglycemia. However, oral administration of extract showed antidiabetic effect with protection of pancreatic beta cells and the induction of glycogen synthesis, no glycogen was deposited in the liver, addressing the secondary effects of diabetes, such as hepatic and renal toxicity. Further discovery revealed that extract elevated antioxidant enzyme expression. Conclusion: Leaf extract from Moringa oleifera reduces blood sugar and lessens the damage caused by hyperglycemia in the pancreas and liver.

辣木叶水提取物对糖尿病 Wistar 大鼠的降血糖作用评估
背景:油辣木叶因其药理作用被用于治疗糖尿病。高血糖患者的β细胞会遭到破坏。然而,尚未开展风险意识研究以确定其安全性。本研究介绍了油辣木叶的抗糖尿病作用,如保护胰岛β细胞和诱导糖原合成,然后探讨了糖尿病的副作用,如肝毒性和肾毒性。研究方法45 只体重为 160±10 克的 Wistar 大鼠分为 9 组。I 组为正常对照组,II 组为用阿脲诱导的糖尿病动物。胰岛素和提取物剂量分别为 200、400 和 800 毫克/千克,用于糖尿病组 III-VI。给第七至第九组的正常动物服用提取物,剂量分别为 200、400 和 800 毫克/千克,持续 28 天。采用标准技术提取组织进行生化和组织学研究。结果与正常对照组(142.75 ± 20.08)相比,糖尿病动物的相对体重下降(95.50 ± 5.50),尿素(对照组为 6.13 ± 0.523,糖尿病组为 29.23 ± 1.267)和肌酐(对照组为 0.70 ± 0.057,糖尿病组为 2.13 ± 0.185)水平升高。肝脏和胰腺的组织学检查也表明,高血糖会导致器官损伤。然而,口服提取物具有抗糖尿病作用,可保护胰腺β细胞并诱导糖原合成,肝脏中没有糖原沉积,从而解决了糖尿病的继发性影响,如肝毒性和肾毒性。进一步研究发现,提取物能提高抗氧化酶的表达。结论油辣木叶提取物能降低血糖,减轻高血糖对胰腺和肝脏造成的损害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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