Cerium oxide nanoparticles-assisted aptasensor for chronic myeloid leukaemia detection.

IF 3.4 Q2 CHEMISTRY, MEDICINAL
ADMET and DMPK Pub Date : 2024-08-18 eCollection Date: 2024-01-01 DOI:10.5599/admet.2404
Yuspian Nur, Muhammad Ihda Hl Zein, Irkham Irkham, Shabarni Gaffar, Toto Subroto, Yeni Wahyuni Hartati
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引用次数: 0

Abstract

Background and purpose: Chronic myeloid leukaemia (CML) is one of the most lethal types of leukaemia and can rapidly progress if not treated properly. Therefore, having an effective diagnostic strategy is crucial. Various methods are available for diagnosis, including electrochemical biosensors with aptamer bioreceptors.

Experimental approach: In this study, we immobilized the KK1D04 aptamer on a screen-printed carbon electrode (SPCE) supported by CeO2 nanoparticles (CeO2NPs) to detect K562 cells, a type of CML cell line. Several parameters were optimized to enhance the aptasensor response using the Box-Behnken experimental design.

Key results: The developed aptasensor demonstrated good performance with a limit of detection (LOD) and limit of quantification (LOQ) of 16 cells/mL and 3,882 cells/mL, respectively, in the K562 cell concentration range of 102 to 106 cells/mL. The optimum experimental conditions were an aptamer concentration of 0.8 ppm, an aptamer incubation time of 36 minutes, and a K562 aptamer-cell incubation time of 13 minutes. The aptasensor also exhibits selectivity for K562 cells compared to Vero cells, THP1 cells, and Raji cells.

Conclusion: The aptasensor in this study demonstrated the potential to detect K562 cells. These results could contribute to the advancement of point-of-care (POC) devices for the detection of CML.

用于检测慢性骨髓性白血病的纳米氧化铈辅助传感器。
背景和目的:慢性髓性白血病(CML)是最致命的白血病类型之一,如果治疗不当,病情会迅速恶化。因此,有效的诊断策略至关重要。目前有多种诊断方法,包括带有适配体生物受体的电化学生物传感器:在这项研究中,我们将 KK1D04 合体固定在由 CeO2 纳米粒子(CeO2NPs)支持的丝网印刷碳电极(SPCE)上,以检测 K562 细胞(一种 CML 细胞系)。采用 Box-Behnken 实验设计优化了多个参数,以提高灵敏度:主要结果:所开发的适配传感器性能良好,在K562细胞浓度为102至106个/毫升的范围内,其检测限(LOD)和定量限(LOQ)分别为16个细胞/毫升和3882个细胞/毫升。最佳实验条件为:适配体浓度为 0.8 ppm,适配体孵育时间为 36 分钟,K562 适配体-细胞孵育时间为 13 分钟。与 Vero 细胞、THP1 细胞和 Raji 细胞相比,该适配体传感器对 K562 细胞也具有选择性:结论:本研究中的适配体传感器具有检测 K562 细胞的潜力。结论:本研究中的适配传感器证明了其检测 K562 细胞的潜力,这些结果将有助于提高用于检测 CML 的护理点 (POC) 设备的水平。
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来源期刊
ADMET and DMPK
ADMET and DMPK Multiple-
CiteScore
4.40
自引率
0.00%
发文量
22
审稿时长
4 weeks
期刊介绍: ADMET and DMPK is an open access journal devoted to the rapid dissemination of new and original scientific results in all areas of absorption, distribution, metabolism, excretion, toxicology and pharmacokinetics of drugs. ADMET and DMPK publishes the following types of contributions: - Original research papers - Feature articles - Review articles - Short communications and Notes - Letters to Editors - Book reviews The scope of the Journal involves, but is not limited to, the following areas: - physico-chemical properties of drugs and methods of their determination - drug permeabilities - drug absorption - drug-drug, drug-protein, drug-membrane and drug-DNA interactions - chemical stability and degradations of drugs - instrumental methods in ADMET - drug metablic processes - routes of administration and excretion of drug - pharmacokinetic/pharmacodynamic study - quantitative structure activity/property relationship - ADME/PK modelling - Toxicology screening - Transporter identification and study
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