Nanodomain-Enhanced Stable and Multifunctional Probes with Near 100% Quantum Yield for Versatile Biosensing.

IF 11.3 1区化学 Q1 CHEMISTRY, PHYSICAL

ACS Catalysis Pub Date : 2024-11-13 Epub Date: 2024-10-31 DOI:10.1021/acs.nanolett.4c04376

Yu Zhang, Niu Feng, Xiaobo Hu, Xufeng Wang, Jiacheng Tao, Zhenguang Ji, Yue Yang, Jimei Ma, Yiping Chen

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Abstract

The preparation of high quantum yield, stable, and multifunctional fluorescent probes is of great significance in the fields of biomedicine and photoelectric sensing. Here, a triphenylamine-based D-π-A fluorescent molecule (TPA-CN) was designed and prepared, demonstrating a fluorescence quantum yield of 88.84%. With a polystyrene nanosphere as the carrier, TPA-CN was encapsulated inside the nanosphere to form intra-nanosphere confining domains. These nanodomain-enhanced fluorescent nanospheres exhibited a fluorescence quantum yield of 98.21%. Using antigen-antibody specificity and the selective catalytic activity of a bioenzyme, with chloramphenicol as a model target, a dual-signal readout biosensor (in fluorescence and colorimetric modes) was designed for ultrasensitive and instrument-free determination. The detection limit was 24 pg/mL within 30 min in fluorescence mode, 38-fold more sensitive and 10-fold faster than that of enzyme linked immunosorbent assays. The nanodomain-enhanced fluorescent probes and dynamic biosensor provide a robust and versatile solution for public health and environmental monitoring needs.

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纳米域增强型稳定多功能探针，量子产率接近 100%，可用于多种生物传感。

制备高量子产率、稳定、多功能的荧光探针在生物医学和光电传感领域具有重要意义。本文设计并制备了一种三苯胺基 D-π-A 荧光分子（TPA-CN），其荧光量子产率高达 88.84%。以聚苯乙烯纳米球为载体，TPA-CN 被封装在纳米球内，形成纳米球内限制域。这些纳米域增强型荧光纳米球的荧光量子产率高达 98.21%。利用抗原-抗体特异性和生物酶的选择性催化活性，以氯霉素为模型目标，设计了一种双信号读出生物传感器（荧光和比色模式），用于超灵敏和无仪器检测。在荧光模式下，30 分钟内的检测限为 24 pg/mL，灵敏度是酶联免疫吸附测定法的 38 倍，检测速度是酶联免疫吸附测定法的 10 倍。纳米结构域增强型荧光探针和动态生物传感器为满足公共卫生和环境监测需求提供了一种稳健的多功能解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Catalysis CHEMISTRY, PHYSICAL-

CiteScore

20.80

自引率

6.20%

发文量

1253

审稿时长

1.5 months

期刊介绍： ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels. The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.