Chemical and metabolic profiling of Amaryllidaceae alkaloids in the bulbs of Narcissus tazetta L. var. chinensis Roem using liquid chromatography–tandem mass spectrometry with data mining strategy

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2024-10-29 DOI:10.1002/rcm.9931

Xinxin Huang, Ting Tan, Yun Luo

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引用次数: 0

Abstract

Rationale

The bulbs of Narcissus tazetta L. var. chinensis Roem (BNTCR) has been used as an herbal medicine for the treatment of carbuncles in China. The previous phytochemical studies indicated that the main active components of BNTCR were Amarllidaceae alkaloids (AAs). However, the comprehensive chemical and metabolic profiling of AAs in BNTCR remains underexplored. Therefore, it is necessary to establish an accurate and rapid approach for chemical characterization and metabolic profiling of AAs in BNTCR.

Methods

The structural elucidation of natural products by ultra-high performance liquid chromatography coupled to quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) with outstanding properties is still limited due to complex mixtures that contain hundreds of compounds. In this study, we employed UHPLC-QTOF-MS/MS with a data mining strategy, including diagnostic fragment ions (DFIs) and neutral loss (NL), for rapid classifying and identifying AAs in BNTCR and prototypes absorbed into the blood of mice after oral administration of BNTCR extract.

Results

As a result, 87 AAs, including 13 narciclasine type, 13 homolycorine type, 17 lycorine type, 12 galanthamine type, 25 haemanthamine type, and 7 tazettine type, were tentatively characterized (6 verified with authentic standards). In addition, a total of 44 prototypes were identified in mice plasma, urine, and fecal samples. Among them, 8 prototypes were found in plasma, 36 in urine, and 40 in feces, respectively. Lycorine, pseudolycorine, homolycorine, 8-O-demetylhomolycorine, and tazettine, as major AAs in BNTCR, were absorbed into the blood.

Conclusions

Our findings provided a crucial step for the elucidation of the active compounds in BNTCR for treatment of carbuncles. In the future, the developed strategy could also be applied for identifying AAs in other herbal medicines from Amarllidaceae.

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采用液相色谱-串联质谱法和数据挖掘策略对水仙鳞茎中的金莲花科生物碱进行化学和代谢分析。

理由：在中国，水仙的鳞茎一直被用作治疗痈肿的中药。以往的植物化学研究表明，BNTCR 的主要活性成分是天南星科生物碱（AAs）。然而，对 BNTCR 中 AAs 的全面化学和代谢特征的研究仍然不足。因此，有必要建立一种准确、快速的方法，对 BNTCR 中的 AAs 进行化学表征和代谢分析：方法：超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-QTOF-MS/MS）具有卓越的性能，但由于含有数百种化合物的复杂混合物，其对天然产物的结构阐释仍然受到限制。在本研究中，我们采用超高效液相色谱-瞬时飞行串联质谱（UHPLC-QTOF-MS/MS）和数据挖掘策略，包括诊断片段离子（DFIs）和中性损失（NL），对 BNTCR 中的 AAs 以及口服 BNTCR 提取物后小鼠血液中吸收的 AAs 原型进行了快速分类和鉴定：结果：初步鉴定了 87 种 AAs，其中包括 13 种 narciclasine 类、13 种 homolycorine 类、17 种 lycorine 类、12 种 galanthamine 类、25 种 haemanthamine 类和 7 种 tazettine 类（其中 6 种已通过真实标准验证）。此外，在小鼠血浆、尿液和粪便样本中共鉴定出 44 种原型。其中，在血浆中发现 8 个原型，在尿液中发现 36 个原型，在粪便中发现 40 个原型。作为 BNTCR 中的主要 AAs，Lycorine、pseudolycorine、homolycorine、8-O-demetylhomolycorine 和 tazettine 被吸收入血：我们的研究结果为阐明 BNTCR 中用于治疗痈肿的活性化合物迈出了关键一步。结论：我们的研究结果为阐明 BNTCR 中用于治疗痈疽的活性化合物迈出了关键的一步。今后，所开发的策略还可用于鉴定天南星科其他中药中的 AAs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.