The OsMAPK5–OsWRKY72 module negatively regulates grain length and grain weight in rice

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fuxiang Wang, Jiexin Lin, Fan Yang, Xiaofeng Chen, Yiyi Liu, Lingnan Yan, Jing Chen, Zonghua Wang, Huaan Xie, Jianfu Zhang, Huibin Xu, Songbiao Chen
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Abstract

Grain size and grain weight are important determinants for grain yield. In this study, we identify a novel OsMAPK5–OsWRKY72 module that negatively regulates grain length and grain weight in rice. We found that loss-of-function of OsMAPK5 leads to larger cell size of the rice spikelet hulls and a significant increase in both grain length and grain weight in an indica variety Minghui 86 (MH86). OsMAPK5 interacts with OsMAPKK3/4/5 and OsWRKY72 and phosphorylates OsWRKY72 at T86 and S88. Similar to the osmapk5 MH86 mutants, the oswrky72 knockout MH86 mutants exhibited larger size of spikelet hull cells and increased grain length and grain weight, whereas the OsWRKY72-overexpression MH86 plants showed opposite phenotypes. OsWRKY72 targets the W-box motifs in the promoter of OsARF6, an auxin response factor involved in auxin signaling. Dual-luciferase reporter assays demonstrated that OsWRKY72 activates OsARF6 expression. The activation effect of the phosphorylation-mimicking OsWRKY72T86D/S88D on OsARF6 expression was significantly enhanced, whereas the effects of the OsWRKY72 phosphorylation-null mutants were significantly reduced. In addition, auxin levels in young panicles of the osmapk5 and oswrky72 mutants were significantly higher than that in the wild-type MH86. Collectively, our study uncovered novel connections of the OsMAPKK3/4/5-OsMAPK5-mediated MAPK signaling, OsWRKY72-mediated transcription regulation, and OsARF6-mediated auxin signaling pathways in regulating grain length and grain weight in an indica-type rice, providing promising targets for molecular breeding of rice varieties with high yield and quality.

OsMAPK5-OsWRKY72 模块负向调节水稻的粒长和粒重。
谷粒大小和谷粒重量是谷物产量的重要决定因素。本研究发现了一个新的 OsMAPK5-OsWRKY72 模块,该模块对水稻的粒长和粒重具有负向调节作用。我们发现,在籼稻品种明恢 86(MH86)中,OsMAPK5 的功能缺失会导致小穗谷壳细胞体积增大,谷粒长度和谷粒重量显著增加。OsMAPK5 与 OsMAPKK3/4/5 和 OsWRKY72 相互作用,并使 OsWRKY72 在 T86 和 S88 处磷酸化。与osmapk5 MH86突变体相似,oswrky72敲除MH86突变体表现出更大的穗壳细胞尺寸以及更长的谷粒长度和谷粒重量,而 OsWRKY72高表达MH86植株则表现出相反的表型。OsWRKY72 靶向 OsARF6 启动子中的 W-box motifs,OsARF6 是一种参与植物生长素信号转导的植物生长素反应因子。双荧光素酶报告实验表明,OsWRKY72 能激活 OsARF6 的表达。磷酸化模拟的 OsWRKY72T86D/S88D 对 OsARF6 表达的激活作用明显增强,而 OsWRKY72 磷酸化缺失突变体的作用则明显减弱。此外,osmapk5和oswrky72突变体幼小圆锥花序中的辅素水平明显高于野生型MH86。总之,我们的研究揭示了OsMAPKK3/4/5-OsMAPK5介导的MAPK信号转导、OsWRKY72介导的转录调控和OsARF6介导的辅素信号转导途径在调控籼型水稻的粒长和粒重中的新联系,为高产优质水稻品种的分子育种提供了前景广阔的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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