Standardization of bone morphometry and mineral density assessments in zebrafish and other small laboratory fishes using x-ray radiography and micro-computed tomography.

IF 5.1 1区 医学 Q1 ENDOCRINOLOGY & METABOLISM
Erika Kague, Ronald Young Kwon, Björn Busse, P Eckhard Witten, David Karasik
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引用次数: 0

Abstract

Zebrafish and other small laboratory fishes are emerging as important animal models for investigating human skeletal development and diseases. In recent years, there has been a notable increase in research publications employing x-ray radiography and micro-computed tomography to analyze the skeletal structures of these animals. However, evaluating bone morphology and mineral density in small laboratory fish poses unique challenges compared to well-established small rodent models. The varied approaches to image acquisition, analysis, and reporting across studies have led to substantial obstacles in interpreting and comparing research findings. This article addresses the urgent need for standardized reporting of parameters and methodologies related to image acquisition and analysis, as well as the adoption of harmonized nomenclature. Furthermore, it offers guidance on anatomical terminology, units of measurement, and the establishment of minimal parameters for reporting, along with comprehensive documentation of methods and algorithms used for acquisition and analysis. We anticipate that adherence to these guidelines will enhance the consistency, reproducibility, and interpretability of reported measurements of bone density and morphometry in small fish models. These advancements are vital for accurately interpreting phenotypes and gene functions, particularly in the context of multi-center studies.

利用 X 射线射线照相术和微型计算机断层扫描技术对斑马鱼和其他实验室小型鱼类的骨骼形态和矿物质密度进行标准化评估。
斑马鱼和其他小型实验鱼类正在成为研究人类骨骼发育和疾病的重要动物模型。近年来,利用 X 射线射线照相术和微型计算机断层扫描技术分析这些动物骨骼结构的研究论文显著增加。然而,与成熟的小型啮齿类动物模型相比,评估实验室小型鱼类的骨骼形态和矿物质密度面临着独特的挑战。不同研究的图像采集、分析和报告方法各不相同,这给解释和比较研究结果造成了巨大障碍。本文探讨了图像采集和分析相关参数和方法的标准化报告以及采用统一术语的迫切需要。此外,文章还就解剖术语、测量单位、建立最低报告参数以及全面记录采集和分析所用的方法和算法提供了指导。我们预计,遵守这些指南将提高小型鱼类模型骨密度和形态测量报告的一致性、可重复性和可解释性。这些进步对于准确解释表型和基因功能至关重要,尤其是在多中心研究的背景下。
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来源期刊
Journal of Bone and Mineral Research
Journal of Bone and Mineral Research 医学-内分泌学与代谢
CiteScore
11.30
自引率
6.50%
发文量
257
审稿时长
2 months
期刊介绍: The Journal of Bone and Mineral Research (JBMR) publishes highly impactful original manuscripts, reviews, and special articles on basic, translational and clinical investigations relevant to the musculoskeletal system and mineral metabolism. Specifically, the journal is interested in original research on the biology and physiology of skeletal tissues, interdisciplinary research spanning the musculoskeletal and other systems, including but not limited to immunology, hematology, energy metabolism, cancer biology, and neurology, and systems biology topics using large scale “-omics” approaches. The journal welcomes clinical research on the pathophysiology, treatment and prevention of osteoporosis and fractures, as well as sarcopenia, disorders of bone and mineral metabolism, and rare or genetically determined bone diseases.
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