Cost-effective chemiresistive biosensor with MWCNT-ZnO nanofibers for early detection of tuberculosis (TB) lipoarabinomannan (LAM) antigen

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-10-29 DOI:10.1007/s00604-024-06780-9

Dinesh R. Rotake, Jitendra B. Zalke, Harsh V. Gechode, Sandhini M. Peshkar, Shiv Govind Singh

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Abstract

The development of an affordable chemiresistive biosensor enhanced with a multi-walled carbon nanotube–zinc oxide (MWCNT-ZnO) nanofiber composite is presented. The sensor leverages the precise interaction between lipoarabinomannan (LAM) tuberculosis (TB) antigens and antibodies to achieve high sensitivity and specificity. The MWCNT-ZnO nanofibers have a larger surface area and better electrical conductivity, which makes it easier for TB antibodies to stick to them. The binding of LAM TB antigens to the fixed Monoclonal Antibody-MBS320597 induces significant resistance changes in the chemiresistive sensor, enabling accurate TB detection. Performance evaluation reveals a linear detection range from 1.0 to 100.0 pg/mL in the lower concentration range and up to 6.0 ng/mL in the higher concentration range, with a sensitivity of 79.750 mA pg mL⁻¹ cm⁻² and a lower limit of detection of 40.54 fg/mL. The sensor exhibits a response time of 102 s. Featuring rapid response time and high sensitivity, this biosensor is ideally suited for point-of-care (PoC) applications. The incorporation of MWCNT-ZnO nanofibers shows great potential for enhancing the development of sensitive and cost-effective TB diagnostic tools, which could play a crucial role in advancing global TB control and management efforts.

Graphical Abstract

查看原文本刊更多论文

采用 MWCNT-ZnO 纳米纤维的高性价比化学电阻生物传感器可用于结核病脂联素甘露聚糖 (LAM) 抗原的早期检测

本文介绍了利用多壁碳纳米管-氧化锌（MWCNT-ZnO）纳米纤维复合材料增强的经济型化学电阻生物传感器的开发情况。该传感器利用脂联素甘露聚糖（LAM）结核病（TB）抗原和抗体之间的精确相互作用实现了高灵敏度和特异性。MWCNT-ZnO 纳米纤维具有更大的表面积和更好的导电性，这使得结核抗体更容易粘附在其上。LAM TB 抗原与固定的单克隆抗体-MBS320597 结合后，化学电阻传感器的电阻会发生显著变化，从而实现准确的 TB 检测。性能评估显示，低浓度范围的线性检测范围为 1.0 至 100.0 pg/mL，高浓度范围可达 6.0 ng/mL，灵敏度为 79.750 mA pg mL-1 cm-2，检测下限为 40.54 fg/mL。该传感器的响应时间为 102 秒。该生物传感器响应时间快、灵敏度高，非常适合用于护理点（PoC）应用。MWCNT-ZnO 纳米纤维的加入显示了加强灵敏度和成本效益型结核病诊断工具开发的巨大潜力，可在推进全球结核病控制和管理工作中发挥关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.