A new immunofluorescence determination of Parkinson's disease biomarkers using silver nanoparticles

IF 6.2 2区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Chao Qin , Jun Xia , Yong Wen, Jun Wang, Chen Zhong
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引用次数: 0

Abstract

Parkinson's disease (PD) is a neurodegenerative condition marked by a steady loss of dopaminergic neurons in the brain's substantia nigra. Prompt identification and tracking of PD progression are essential for prompt intervention and efficient PD care. In this study, we developed an immunofluorescence detection approach for α-synuclein (α-syn), a critical biomarker associated with PD, that is both extremely sensitive and specific. Using polyethylene glycol (PEG)-functionalized magnetic beads (MBs) and an Ag+ fluorescence probe (Ag+-FP) based on Rhodamine 6 G, the suggested method makes use of an immunofluorescence detection system. The system's workings are based on antigen-antibody complexes. Identified as Ab1-MBs@α-syn@Ab2-Ag NPs, the immuno-complexes encapsulate α-synuclein between anti-α-synuclein antibodies (Ab1) fixed on amino-MBs and Ag Nanoparticles functionalized with matching Ab2. α-synuclein detection was accomplished at a limit of less than 8 pg/mL through optimization of pH, reaction duration, and antibody concentration. The method showed very little cross-reactivity with other widely used biomarkers and a high specificity. The system showed a linear range of 524.8 ng/mL to 0.2 ng/mL. The results, which showed recovery values ranging from 97.00 % to 99.57 % and were consistent with those obtained using a commercial ELISA kit, indicated the system's potential for clinical applications in the diagnosis and monitoring of PD.
利用银纳米粒子对帕金森病生物标记物进行免疫荧光测定的新方法
帕金森病(PD)是一种神经退行性疾病,以大脑黑质中多巴胺能神经元的持续丧失为特征。及时发现和跟踪帕金森病的进展对于及时干预和有效治疗帕金森病至关重要。在这项研究中,我们开发了一种免疫荧光检测α-突触核蛋白(α-syn)的方法,这是一种与帕金森病相关的重要生物标记物,具有极高的灵敏度和特异性。所建议的方法使用聚乙二醇(PEG)功能化磁珠(MBs)和基于罗丹明6 G的Ag+荧光探针(Ag+-FP),利用免疫荧光检测系统。该系统的工作原理基于抗原-抗体复合物。这种免疫复合物被称为 Ab1-MBs@α-syn@Ab2-Ag NPs,它将固定在氨基-MBs 上的抗α-突触核蛋白抗体(Ab1)与用匹配的 Ab2 功能化的 Ag 纳米粒子之间的α-突触核蛋白包裹在一起。该方法与其他广泛使用的生物标记物几乎没有交叉反应,而且特异性很高。该系统的线性范围为 524.8 纳克/毫升至 0.2 纳克/毫升。结果显示回收率在97.00%到99.57%之间,与使用商业ELISA试剂盒获得的结果一致,这表明该系统在诊断和监测PD的临床应用方面具有潜力。
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来源期刊
alexandria engineering journal
alexandria engineering journal Engineering-General Engineering
CiteScore
11.20
自引率
4.40%
发文量
1015
审稿时长
43 days
期刊介绍: Alexandria Engineering Journal is an international journal devoted to publishing high quality papers in the field of engineering and applied science. Alexandria Engineering Journal is cited in the Engineering Information Services (EIS) and the Chemical Abstracts (CA). The papers published in Alexandria Engineering Journal are grouped into five sections, according to the following classification: • Mechanical, Production, Marine and Textile Engineering • Electrical Engineering, Computer Science and Nuclear Engineering • Civil and Architecture Engineering • Chemical Engineering and Applied Sciences • Environmental Engineering
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