Genome-wide characterization and comparative transcriptomics unravel CpMADS47 as a positive regulator during fruit ripening and softening in Chinese cherry

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-10-29 DOI:10.1016/j.postharvbio.2024.113287

Tai Tian , Shiqing Yin , Fengting Huang , Longqiang Feng , Yan Ma , Hao Wang , Jing Zhang , Wen He , Yuanxiu Lin , Yunting Zhang , Mengyao Li , Zhiwei Wu , Yong Zhang , Ya Luo , Haoru Tang , Qing Chen , Xiaorong Wang , Yan Wang

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引用次数: 0

Abstract

Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don] (syn. Prunus pseudocerasus Lindl.) is an economically important fruit crop native to China. The fruits are prone to softening and rotting after harvest, which significantly limits their marketability and hinders its rapid development throughout China. The MADS-box gene family, particularly the SEP subfamily, plays a crucial role in governing fruit ripening and softening. However, the molecular mechanisms underlying fruit ripening and softening in Chinese cherry remains unclear. Herein, we identified 92 MADS genes from the Chinese cherry genome and analyzed their physicochemical characteristics, chromosomal localization, phylogeny, gene structures, covariance, and cis-acting elements. Many cis-elements in the promoters of CpMADSs are implicated in fruit development, ripening and stress response. Using comparative transcriptomics and RT-qPCR analysis, we identified a key gene, CpMADS47, as a positive regulator of cherry fruit ripening. CpMADS47 is localized in both the nucleus and cell membrane and shows highly expression in flowers and mature fruits. Transient overexpression of CpMADS47 in cherry fruit demonstrated its role in mediating fruit ripening and softening by promoting reduction in fruit firmness, anthocyanin accumulation, depolymerization of cell wall components, enhancement of cell wall degradation enzyme activity, and ABA biosynthesis. Conversely, silencing CpMADS47 generated the opposite effect. Yeast one-hybrid and dual-luciferase assays revealed that the CpMADS47 targets the promoters of cell wall degrading genes (CpPME3 and CpXTH31) and ABA signal transduction genes (CpPP2C12), thereby activating their transcription and promoting cherry fruit ripening. In summary, this study enriches our understanding of the transcriptional regulation of fruit ripening and softening in Chinese cherry.

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全基因组表征和比较转录组学揭示了 CpMADS47 在中国樱桃果实成熟和软化过程中的积极调控作用

中国樱桃[Cerasus pseudocerasus (Lindl.) G.Don]（同属 Prunus pseudocerasus Lindl.）是原产于中国的一种经济上重要的水果作物。果实采收后容易软化和腐烂，极大地限制了其销路，阻碍了其在中国各地的快速发展。MADS-box 基因家族，尤其是 SEP 亚家族，在果实成熟和软化过程中起着至关重要的作用。然而，中国樱桃果实成熟和软化的分子机制仍不清楚。在此，我们从中国樱桃基因组中鉴定了92个MADS基因，并分析了它们的理化特征、染色体定位、系统发育、基因结构、共变性和顺式作用元件。CpMADS 启动子中的许多顺式元件与果实发育、成熟和胁迫响应有关。通过比较转录组学和 RT-qPCR 分析，我们发现了一个关键基因 CpMADS47，它是樱桃果实成熟的正调控因子。CpMADS47 定位于细胞核和细胞膜，在花和成熟果实中均有高表达。CpMADS47 在樱桃果实中的瞬时过表达表明，它通过促进果实硬度下降、花青素积累、细胞壁成分解聚、细胞壁降解酶活性增强和 ABA 生物合成等作用，介导果实成熟和软化。相反，沉默 CpMADS47 则会产生相反的效果。酵母单杂交和双荧光素酶测定显示，CpMADS47靶向细胞壁降解基因（CpPME3 和 CpXTH31）和 ABA 信号转导基因（CpPP2C12）的启动子，从而激活它们的转录，促进樱桃果实成熟。总之，本研究丰富了我们对中国樱桃果实成熟和软化转录调控的认识。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.