UV-B radiation mitigates oxidative stress damage in postharvest Agaricus bisporus by modulating the antioxidant defense system

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-10-19 DOI:10.1016/j.postharvbio.2024.113266

Xueli Shang , Shiqi Bai , Liang Wen , Alfred Mugambi Mariga , Ning Ma , Donglu Fang , Wenjian Yang , Qiuhui Hu , Fei Pei

{"title":"UV-B radiation mitigates oxidative stress damage in postharvest Agaricus bisporus by modulating the antioxidant defense system","authors":"Xueli Shang , Shiqi Bai , Liang Wen , Alfred Mugambi Mariga , Ning Ma , Donglu Fang , Wenjian Yang , Qiuhui Hu , Fei Pei","doi":"10.1016/j.postharvbio.2024.113266","DOIUrl":null,"url":null,"abstract":"<div><div>Agaricus bisporus (A. bisporus) has fragile tissues and is highly susceptible to post-harvest decay and spoilage, which affecting the development of the industry. Ultraviolet B (UV-B) irradiation, as a typical irradiation preservation technology, is effective in inducing the production of endogenous metabolic substances in organisms and enhancing their level of resistance. The objective of this study was to investigate the mechanism of activation of the antioxidant defence system in A. bisporus by UV-B irradiation, utilising a range of UV-B irradiation doses (0, 25, 50 and 100 kJ m−2). In this study we found that 50 kJ m−2 UV-B irradiation effectively delayed the accumulation of reactive oxygen species (ROS), inhibited NADPH oxidase (NOX) activity and the expression of Rbohf, PXMP2, PXMP4, APO, and MPV17. Moreover, it could increase the accumulation of ascorbate (AsA) and glutathione (GSH), enhance the activities of ascorbate peroxidase (APX) and glutathione peroxidase (GSH-PX), and it also effectively induce catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) activities and up-regulate the expression levels of related genes. In addition, we found that UV-B irradiation upregulated the expression of UVR8 and suppressed the expression of PEX5, PEX11 and PMD1. These results suggest that 50 kJ m−2 UV-B irradiation could stimulate the UV Resistance Loucs 8 (UVR8) receptor, regulate peroxisome proliferation, and enhance the ability of A. bisporus to resist oxidative stress, thereby maintaining the cellular redox homeostasis, this provides a new strategy for the study of extended postharvest storage stability of A. bisporus.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113266"},"PeriodicalIF":6.4000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424005118","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Agaricus bisporus (A. bisporus) has fragile tissues and is highly susceptible to post-harvest decay and spoilage, which affecting the development of the industry. Ultraviolet B (UV-B) irradiation, as a typical irradiation preservation technology, is effective in inducing the production of endogenous metabolic substances in organisms and enhancing their level of resistance. The objective of this study was to investigate the mechanism of activation of the antioxidant defence system in A. bisporus by UV-B irradiation, utilising a range of UV-B irradiation doses (0, 25, 50 and 100 kJ m⁻²). In this study we found that 50 kJ m⁻² UV-B irradiation effectively delayed the accumulation of reactive oxygen species (ROS), inhibited NADPH oxidase (NOX) activity and the expression of Rbohf, PXMP2, PXMP4, APO, and MPV17. Moreover, it could increase the accumulation of ascorbate (AsA) and glutathione (GSH), enhance the activities of ascorbate peroxidase (APX) and glutathione peroxidase (GSH-PX), and it also effectively induce catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) activities and up-regulate the expression levels of related genes. In addition, we found that UV-B irradiation upregulated the expression of UVR8 and suppressed the expression of PEX5, PEX11 and PMD1. These results suggest that 50 kJ m⁻² UV-B irradiation could stimulate the UV Resistance Loucs 8 (UVR8) receptor, regulate peroxisome proliferation, and enhance the ability of A. bisporus to resist oxidative stress, thereby maintaining the cellular redox homeostasis, this provides a new strategy for the study of extended postharvest storage stability of A. bisporus.

查看原文本刊更多论文

紫外线-B 辐射通过调节抗氧化防御系统减轻收获后双孢蘑菇的氧化应激损伤

双孢蘑菇（A. bisporus）组织脆弱，收获后极易腐烂变质，影响产业发展。紫外线 B（UV-B）辐照作为一种典型的辐照保鲜技术，能有效诱导生物体产生内源代谢物质，提高其抗性水平。本研究的目的是利用一系列紫外线-B 照射剂量（0、25、50 和 100 kJ m-2）研究紫外线-B 照射激活双孢菇抗氧化防御系统的机制。在这项研究中，我们发现 50 kJ m-2 紫外线-B 照射能有效延缓活性氧（ROS）的积累，抑制 NADPH 氧化酶（NOX）的活性以及 Rbohf、PXMP2、PXMP4、APO 和 MPV17 的表达。此外，它还能增加抗坏血酸（AsA）和谷胱甘肽（GSH）的积累，提高抗坏血酸过氧化物酶（APX）和谷胱甘肽过氧化物酶（GSH-PX）的活性，还能有效诱导过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性，并上调相关基因的表达水平。此外，我们还发现 UV-B 照射会上调 UVR8 的表达，抑制 PEX5、PEX11 和 PMD1 的表达。这些结果表明，50 kJ m-2 UV-B 照射可刺激紫外线抗性路cs 8（UVR8）受体，调节过氧化物酶体增殖，增强双孢蘑菇抵抗氧化应激的能力，从而维持细胞氧化还原平衡，这为研究延长双孢蘑菇采后贮藏稳定性提供了新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.