Improvement in bioactivity, hardness and friction resistance of 3 % manganese-doped hydroxyapatite coated on alumina using radio frequency magnetron sputtering

IF 5.3 2区材料科学 Q1 MATERIALS SCIENCE, COATINGS & FILMS

Surface & Coatings Technology Pub Date : 2024-10-30 DOI:10.1016/j.surfcoat.2024.131481

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引用次数: 0

Abstract

Hydroxyapatite (HAP) is a common hard tissue implant material known for its superior biocompatibility and osteoconductivity. However, its poor mechanical strength, brittleness and slow degradation limit the applications. This study explores the enhancement of HAP mechanical properties and bioactivity by coating 3 wt% manganese-doped HAP (Mn-HAP) on another inert biomaterial alumina (Mn-HAP/Al₂O₃) substrates using the RF magnetron sputtering technique. Characterization of these samples was performed using Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X-ray Spectroscopy (EDS), Grazing Incidence X-ray Diffraction (GIXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Brunauer-Emmett-Teller (BET) techniques. Mechanical property was assessed through Vicker's hardness and adhesion of the film was studied by scratch testing. Corrosion resistance was evaluated using Tafel plots in Ringer's solution by Electrochemical analyser (ECA), and dielectric properties were measured using Impedance analyser. Biocompatibility was examined by wettability tests, thrombogenicity, antioxidant test, antimicrobial investigation and MTT [3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. The results show that Mn-HAP/Al₂O₃ coatings exhibit superior properties as compared to pure HAP, alumina, and HAP/Al₂O₃. Mn-HAP showed enhanced crystallinity and grain refinement, leading to improved hardness of 1198 HV for Mn-HAP/Al₂O₃ as compared to 39.84 HV for pure HAP and 1028 HV for HAP/Al₂O₃. The friction coefficient was found to be best in the Mn-HAP/Al₂O₃ sample. Corrosion rate significantly decreases in Mn-HAP/Al₂O₃ (1.63

\pm

0.28) mmpy after coating on alumina. In vitro studies demonstrated enhanced cell attachment, proliferation, and differentiation after Mn-HAP coating on alumina. Antimicrobial tests revealed improved resistance against E. coli and S. aureus, with Mn-HAP/Al₂O₃ showing a larger zone of inhibition. The study concludes that 3 wt% Mn-HAP coatings deposited by RF magnetron sputtering hold great promise for enhancing the performance and longevity of hard tissue implants, paving the way for advanced biomedical applications.

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利用射频磁控溅射技术提高氧化铝上 3%掺锰羟基磷灰石涂层的生物活性、硬度和耐摩擦性

羟基磷灰石（HAP）是一种常见的硬组织植入材料，以其优异的生物相容性和骨传导性而闻名。然而，其机械强度差、脆性大、降解慢等特点限制了其应用。本研究采用射频磁控溅射技术，在另一种惰性生物材料氧化铝（Mn-HAP/Al2O3）基底上涂覆 3 wt%的锰掺杂 HAP（Mn-HAP），探索如何提高 HAP 的机械性能和生物活性。使用场发射扫描电子显微镜 (FESEM)、能量色散 X 射线光谱仪 (EDS)、掠入射 X 射线衍射 (GIXRD)、傅立叶变换红外光谱仪 (FTIR) 和 Brunauer-Emmett-Teller (BET) 技术对这些样品进行了表征。机械性能通过维氏硬度进行评估，薄膜的附着力则通过划痕测试进行研究。电化学分析仪（ECA）利用林格氏溶液中的塔菲尔图评估了抗腐蚀性，并利用阻抗分析仪测量了介电性能。通过湿润性测试、血栓形成性、抗氧化测试、抗菌调查和 MTT[3-（4, 5-二甲基噻唑-2-基）-2, 5-二苯基溴化四氮唑]试验检验了生物相容性。结果表明，与纯 HAP、氧化铝和 HAP/Al2O3 相比，Mn-HAP/Al2O3 涂层具有更优越的性能。Mn-HAP 显示出更高的结晶度和晶粒细化度，从而使 Mn-HAP/Al2O3 的硬度提高到 1198 HV，而纯 HAP 为 39.84 HV，HAP/Al2O3 为 1028 HV。Mn-HAP/Al2O3 样品的摩擦系数最佳。在氧化铝上镀膜后，Mn-HAP/Al2O3 的腐蚀速率明显降低（1.63 ± 0.28）mmpy。体外研究表明，在氧化铝上涂覆 Mn-HAP 后，细胞的附着、增殖和分化能力得到增强。抗菌测试表明，Mn-HAP/Al2O3 对大肠杆菌和金黄色葡萄球菌的抗性有所提高，抑制区更大。研究得出结论，通过射频磁控溅射沉积的 3 wt% Mn-HAP 涂层有望提高硬组织植入物的性能和寿命，为先进的生物医学应用铺平道路。

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来源期刊

Surface & Coatings Technology 工程技术-材料科学：膜

CiteScore

10.00

自引率

11.10%

发文量

921

审稿时长

19 days

期刊介绍： Surface and Coatings Technology is an international archival journal publishing scientific papers on significant developments in surface and interface engineering to modify and improve the surface properties of materials for protection in demanding contact conditions or aggressive environments, or for enhanced functional performance. Contributions range from original scientific articles concerned with fundamental and applied aspects of research or direct applications of metallic, inorganic, organic and composite coatings, to invited reviews of current technology in specific areas. Papers submitted to this journal are expected to be in line with the following aspects in processes, and properties/performance: A. Processes: Physical and chemical vapour deposition techniques, thermal and plasma spraying, surface modification by directed energy techniques such as ion, electron and laser beams, thermo-chemical treatment, wet chemical and electrochemical processes such as plating, sol-gel coating, anodization, plasma electrolytic oxidation, etc., but excluding painting. B. Properties/performance: friction performance, wear resistance (e.g., abrasion, erosion, fretting, etc), corrosion and oxidation resistance, thermal protection, diffusion resistance, hydrophilicity/hydrophobicity, and properties relevant to smart materials behaviour and enhanced multifunctional performance for environmental, energy and medical applications, but excluding device aspects.