Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-10-19 DOI:10.1016/j.bej.2024.109534

Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto

{"title":"Adsorption characteristics of virus-mimetic fluorescent nanoparticles on polymer fiber material surfaces","authors":"Rina Uchida , Ayuri Mitsuno , Tomohiro Komatsu , Chisato Sakamoto , Satoshi Amaya , Satoshi Migita , Eiichiro Takamura , Hiroaki Sakamoto","doi":"10.1016/j.bej.2024.109534","DOIUrl":null,"url":null,"abstract":"<div><div>Contact infection, whereby the virus is transmitted through objects to which it adheres, affects the spread of infection. Although inactivation with alcohol is commonly used, the virus may adhere to the surface again after removal. It is necessary to take measures against direct and contact infection to control the spread of viral infection. However, the type of material the viruses adsorb onto is not fully understood. The objective in the present study is to elucidate virus adsorption behavior to suppress the indirect spread of infection. We examined for materials that are difficult for viruses to adhere to and investigated their adsorption mechanisms to control the spread of infection through contact infection. In this study, spike protein-modified fluorescent nanoparticles (SFNs) were designed and fabricated by modifying the surface of fluorescent particles with the spike S1 protein, which is expected to first contact and adsorb onto the material surface. Purpose of the present study is to analyze the influence of material characteristics on virus adhesion using SFNs. SFNs were adsorbed onto Nylon, polyester (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), acrylic and rayon, and their fluorescence intensities and adsorption characteristics were compared by scanning electron microscopy (SEM) analysis and fluorescence analysis. Principal component analysis showed that virus adsorption was more sensitive to coarseness for PTFE than for other fibers.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"213 ","pages":"Article 109534"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24003218","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Contact infection, whereby the virus is transmitted through objects to which it adheres, affects the spread of infection. Although inactivation with alcohol is commonly used, the virus may adhere to the surface again after removal. It is necessary to take measures against direct and contact infection to control the spread of viral infection. However, the type of material the viruses adsorb onto is not fully understood. The objective in the present study is to elucidate virus adsorption behavior to suppress the indirect spread of infection. We examined for materials that are difficult for viruses to adhere to and investigated their adsorption mechanisms to control the spread of infection through contact infection. In this study, spike protein-modified fluorescent nanoparticles (SFNs) were designed and fabricated by modifying the surface of fluorescent particles with the spike S1 protein, which is expected to first contact and adsorb onto the material surface. Purpose of the present study is to analyze the influence of material characteristics on virus adhesion using SFNs. SFNs were adsorbed onto Nylon, polyester (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), acrylic and rayon, and their fluorescence intensities and adsorption characteristics were compared by scanning electron microscopy (SEM) analysis and fluorescence analysis. Principal component analysis showed that virus adsorption was more sensitive to coarseness for PTFE than for other fibers.

查看原文本刊更多论文

仿病毒荧光纳米粒子在聚合物纤维材料表面的吸附特性

接触传染，即病毒通过其附着的物体传播，会影响感染的扩散。虽然常用酒精灭活，但清除后病毒可能会再次附着在物体表面。有必要采取措施防止直接感染和接触感染，以控制病毒感染的传播。然而，人们对病毒吸附在哪种材料上并不完全了解。本研究的目的是阐明病毒的吸附行为，以抑制感染的间接传播。我们研究了病毒难以吸附的材料，并调查了它们的吸附机制，以控制通过接触感染的传播。本研究设计并制造了尖峰蛋白修饰的荧光纳米粒子（SFNs），方法是用尖峰 S1 蛋白修饰荧光粒子表面，使其首先接触并吸附在材料表面。本研究的目的是利用 SFNs 分析材料特性对病毒吸附的影响。研究人员将 SFNs 吸附在尼龙、聚酯（PET）、聚丙烯（PP）、聚四氟乙烯（PTFE）、腈纶和人造丝上，并通过扫描电子显微镜（SEM）分析和荧光分析比较了它们的荧光强度和吸附特性。主成分分析表明，与其他纤维相比，聚四氟乙烯纤维的病毒吸附对粗细度更为敏感。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.