Impact of altitudinal gradients on chemical profiling and pesticidal activities of Hedychium spicatum Sm

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2024-10-25 DOI:10.1016/j.bse.2024.104914

Avneesh Rawat , Om Prakash , Ravendra Kumar , Satya Kumar , R.M. Srivastava , Mamta Latwal , Ganesh Pandey

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引用次数: 0

Abstract

Hedychium spicatum Sm. is an endangered herb which grows preferably in humid tropics and subtropics known for its distinct camphoraceous flavour and medicinal properties. The essential oils of H. spicatum rhizomes from six different natural habitats at different altitudes in Uttarakhand, India were analyzed. Twenty compounds were identified, accounting for 87.4–98.5% of the essential oils, with 1,8-cineole (33.4–41.9%), α-terpinene (39.6%), camphor (31.4%), and linalool (29.9%), being the prevalent major constituents of all accessions. HS-ALM (H. spicatum rhizomes Almora essential oil), HS-DDN (H. spicatum rhizomes Dehradun essential oil), HS-PAU (H. spicatum rhizomes Pauri essential oil), HS-TEH (H. spicatum rhizomes Tehri essential oil), and HS-USN (H. spicatum rhizomes Udham Singh Nagar essential oil) were dominated by oxygenated monoterpenes with 43.1–74.0%, respectively. In contrast, in HS-PIT (H. spicatum rhizomes Pithoragarh essential oil), hydrogenated monoterpenes dominated 57.8% of the total oil constituents. All accessions were assessed for significant nematicidal potential against Meloidogyne incognita and insecticidal potential against Spodoptera litura. The proteins/enzymes used for validation using docking studies were acetylcholinesterase (PBD ID: IC2O) and carboxylesterase (PDB ID: 1CI8). The study produced significant outcomes, showing the binding energy estimation of tested oil components to active sites supported the structure-activity relationship with bioinformatics tools.

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海拔梯度对刺五加（Hedychium spicatum Sm）化学特征和杀虫活性的影响

Hedychium spicatum Sm. 是一种濒危草本植物，主要生长在潮湿的热带和亚热带地区，以其独特的樟脑味和药用特性而闻名。研究人员分析了来自印度北阿坎德邦不同海拔地区六个不同自然栖息地的 H. spicatum 根茎的精油。鉴定出 20 种化合物，占精油的 87.4-98.5%，其中 1,8-蒎烯（33.4-41.9%）、α-萜品烯（39.6%）、樟脑（31.4%）和芳樟醇（29.9%）是所有品种的主要成分。HS-ALM（H. spicatum rhizomes Almora 精油）、HS-DDN（H. spicatum rhizomes Dehradun 精油）、HS-PAU（H. spicatum rhizomes Pauri 精油）、HS-TEH（H. spicatum rhizomes Tehri 精油）和 HS-USN（H. spicatum rhizomes Udham Singh Nagar 精油）以含氧单萜为主，分别占 43.1-74.0% 。相反，在 HS-PIT（H. spicatum rhizomes Pithoragarh 精油）中，氢化单萜占精油总成分的 57.8%。对所有品种都进行了评估，以确定其对 Meloidogyne incognita 的杀线虫潜力和对 Spodoptera litura 的杀虫潜力。利用对接研究进行验证的蛋白质/酶是乙酰胆碱酯酶（PBD ID：IC2O）和羧基酯酶（PDB ID：1CI8）。研究结果表明，通过生物信息学工具对测试油成分与活性位点的结合能进行估算，支持了结构-活性关系。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.