Development of method and analysis protocol for fatty acids derivatives of castor oil by using gas chromatography-mass spectrometry

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-01 DOI:10.1016/j.bcab.2024.103408

Amzad Hossain, Akansha Pal, Shadeed Salem

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引用次数: 0

Abstract

Castor oil is a vegetable oil extracted from castor beans, known for its diverse applications across the chemical industry, food industry, skincare products, biodiesel components, and pharmaceuticals. Analyzing the fatty acid composition of castor oil using Gas Chromatography-Mass Spectrometry (GC-MS) can be complex due to various factors inherent to fatty acids and the oil's composition. Therefore, the goal of this study is to effectively separate and analyze the major fatty acids present in castor oil using GC-MS. Castor oil was methylated using alcoholic potassium hydroxide, then neutralized with salicylic acid and centrifuged. The supernatant was diluted and injected into the GC-MS with a developed temperature program. The results indicated that castor oil contains five fatty acids: ricinoleic acid methyl ester, oleic acid methyl ester, linoleic acid methyl ester, stearic acid methyl ester, and palmitic acid methyl ester, with respective concentrations of 87.63%, 1.81%, 6.57%, 2.93%, and 1.07%. Among these, methyl ricinoleate was the most abundant at approximately 87.36%, while palmitic acid methyl ester had the lowest concentration at 1.07%. The order of fatty acids detected was methyl ricinoleate > linoleic acid > stearic acid > oleic acid methyl ester > palmitic acid. To our knowledge, this is the first report of an easy and efficient method for the analysis of castor oil. In conclusion, the developed methylation of castor oil and gas chromatography temperature program are suitable for the routine analysis of castor oil.

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利用气相色谱-质谱法开发蓖麻油脂肪酸衍生物的方法和分析规程

蓖麻油是从蓖麻中提取的一种植物油，因其在化学工业、食品工业、护肤品、生物柴油成分和制药等领域的广泛应用而闻名。使用气相色谱-质谱法（GC-MS）分析蓖麻油的脂肪酸组成可能会很复杂，这是因为脂肪酸和油的组成存在各种固有因素。因此，本研究的目标是利用气相色谱-质谱法有效地分离和分析蓖麻油中的主要脂肪酸。蓖麻油使用氢氧化钾酒精进行甲基化，然后用水杨酸中和并离心。上清液经过稀释后，按照设定的温度程序注入气相色谱-质谱仪。结果表明，蓖麻油中含有五种脂肪酸：蓖麻油酸甲酯、油酸甲酯、亚油酸甲酯、硬脂酸甲酯和棕榈酸甲酯，其浓度分别为 87.63%、1.81%、6.57%、2.93% 和 1.07%。其中，蓖麻油酸甲酯的含量最高，约为 87.36%，而棕榈酸甲酯的含量最低，仅为 1.07%。检测到的脂肪酸顺序为：蓖麻油酸甲酯；亚油酸；硬脂酸；油酸甲酯；棕榈酸。据我们所知，这是首次报道用于分析蓖麻油的简便而高效的方法。总之，所开发的蓖麻油甲基化和气相色谱温度程序适用于蓖麻油的常规分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.