Enhanced catalytic activity and reusability of sucrose phosphorylase@magnetic nanoparticles by surface-coating amorphous ZIF-67

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-10-16 DOI:10.1016/j.bej.2024.109533

Zhendong Liu , Haichang Xu , Bin Wei , Hao Liang

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引用次数: 0

Abstract

Sucrose phosphorylase (SPase) is a highly efficient glycosyltransferase which has a wide range of substrate specificity and excellent application prospects in the cosmetics, food, and medicine fields. The application of free SPase is limited due to its high cost, poor stability and poor reusability. Immobilization of enzymes can solve these problems. In this study, SPase was firstly immobilized by magnetic nanoparticles (MNPs) and surface-coating amorphous ZIF-67 for enhancing catalytical activity and reusability. ZIF-67@SPase@MNPs with MNPs core and ZIF-67 shell was characterized by TEM, SEM, XRD, FT-IR and VSM. Compared with free SPase, the catalytic activity of ZIF-67@SPase@MNPs increased by 30 %. Coated with amorphous ZIF-67, the immobilized enzyme retained 70 % relative activity after 12 cycles and 80 % relative activity after 15 days of storage. In addition, ZIF-67@SPase@MNPs had strong magnetic properties and the saturation magnetization was 52.07 emu/g. Surface-coating amorphous ZIF-67 on SPase@MNPs is a promising method for immobilizing enzymes, which can improve catalytic activity and reusability, therefore showing great application potential in biocatalysts and product separation. It has a good application prospect in the production of high viscosity glycosides.

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通过表面包覆无定形 ZIF-67 增强蔗糖磷酸化酶@磁性纳米粒子的催化活性和重复使用性

蔗糖磷酸化酶（SPase）是一种高效的糖基转移酶，具有广泛的底物特异性，在化妆品、食品和医药领域具有良好的应用前景。由于游离 SPase 成本高、稳定性差、重复利用率低，其应用受到限制。酶的固定化可以解决这些问题。本研究首先利用磁性纳米颗粒（MNPs）固定化 SPase，然后在其表面包覆无定形 ZIF-67，以提高其催化活性和可重复使用性。研究人员利用 TEM、SEM、XRD、FT-IR 和 VSM 对以 MNPs 为核心、ZIF-67 为外壳的 ZIF-67@SPase@MNPs 进行了表征。与游离 SPase 相比，ZIF-67@SPase@MNPs 的催化活性提高了 30%。包覆了无定形 ZIF-67 的固定化酶在 12 个循环后保持了 70% 的相对活性，在储存 15 天后保持了 80% 的相对活性。此外，ZIF-67@SPase@MNPs 还具有很强的磁性，其饱和磁化率为 52.07 emu/g。在 SPase@MNPs 表面包覆无定形 ZIF-67 是一种很有前景的固定化酶的方法，可以提高催化活性和重复利用率，因此在生物催化剂和产物分离方面具有很大的应用潜力。它在高粘度糖苷的生产中具有良好的应用前景。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.