Structural analysis and accelerating wound healing function of a novel galactosylated glycosaminoglycan from the snail Helix lucorum

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2024-10-21 DOI:10.1016/j.carbpol.2024.122900

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引用次数: 0

Abstract

Diabetic foot ulcers (DFUs) as a nonhealing wound remain a clinical challenge, and the development of pro-healing and cost-effective drugs is in urgent need. Herein, we reported a novel galactosylated glycosaminoglycan (GAG) from the snail Helix lucorum, as an effective pro-healing compound. The snail GAG is composed of a heparan sulfate-like main chain and galactose side chains at C-3 of GlcNAc residue. Its main chain has a repeating disaccharide structure of → 4)-α-D-GlcNAc-(1 → 4)-α-L-IdoA_2S(1 →. This is the first example of glycosaminoglycan with galactose branches from mollusks. Pharmacological experiments showed that the H. lucorum GAG significantly promoted skin wound healing in both healthy and diabetic mice by accelerating granulation tissue regeneration, angiogenesis, and collagen deposition. The distinctive galactosylated substitution may play an important role on its pro-healing activity. Our discovery enriches the diversity of non-anticoagulant heparan sulfate-like glycosaminoglycans, and provides a potential candidate of pro-healing drug for treating diabetic wound.

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来自蜗牛 Helix lucorum 的新型半乳糖基化氨基糖的结构分析和加速伤口愈合功能

糖尿病足溃疡（DFUs）作为一种不愈合伤口，仍然是临床上的一大难题，因此急需开发促进愈合且经济有效的药物。在此，我们报告了一种来自蜗牛 Helix lucorum 的新型半乳糖基化氨基糖（GAG），它是一种有效的促愈合化合物。蜗牛 GAG 由硫酸肝素样主链和位于 GlcNAc 残基 C-3 的半乳糖侧链组成。其主链的重复双糖结构为 → 4)-α-D-GlcNAc-(1 → 4)-α-L-IdoA2S(1 →)。这是软体动物中第一个带有半乳糖分支的氨基糖。药理实验表明，H. lucorum GAG 可通过加速肉芽组织再生、血管生成和胶原沉积，显著促进健康小鼠和糖尿病小鼠的皮肤伤口愈合。独特的半乳糖基取代可能对其促进伤口愈合的活性起着重要作用。我们的发现丰富了非抗凝血硫酸肝素类氨基糖的多样性，为治疗糖尿病伤口提供了一种潜在的促进愈合药物候选物。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.